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Occurrence of diarrhoeagenic Escherichia coli virulence genes in water and bed sediments of a river used by communities in Gauteng, South Africa

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dc.contributor.author Abia, ALK
dc.contributor.author Ubomba-Jaswa, Eunice
dc.contributor.author Momba, MNB
dc.date.accessioned 2017-07-28T09:10:03Z
dc.date.available 2017-07-28T09:10:03Z
dc.date.issued 2016-08
dc.identifier.citation Abia, A.L.K., Ubomba-Jaswa, E. and Momba, M.N.B. 2016. Occurrence of diarrhoeagenic Escherichia coli virulence genes in water and bed sediments of a river used by communities in Gauteng, South Africa. Environmental Science and Pollution Research, vol. 23(15): 15665–15674. DOI: 10.1007/s11356-016-6762-6 en_US
dc.identifier.issn 0944-1344
dc.identifier.uri DOI: 10.1007/s11356-016-6762-6
dc.identifier.uri https://link.springer.com/article/10.1007%2Fs11356-016-6762-6
dc.identifier.uri http://hdl.handle.net/10204/9377
dc.description Copyright: 2016 Springer. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. en_US
dc.description.abstract In most developing countries, especially in Southern Africa, little is known about the presence of diarrhoeagenic Escherichia coli (DEC) pathotypes in riverbed sediments. The present study sought to investigate the presence of DEC virulence genes in riverbed sediments of the Apies River, a river used by many communities in Gauteng, South Africa. Water and sediment samples were collected from the river between July and August 2013 (dry season) and also between January and February 2014 (wet season) following standard procedures. Isolation of E. coli was done using the Colilert®-18 Quanti-Tray® 2000 system. DNA was extracted from E. coli isolates using the InstaGene™ matrix from Bio-Rad and used as template DNA for real-time PCR. Water pH, temperature, dissolved oxygen, electrical conductivity and turbidity were measured in situ. Over 59% of 180 samples analysed were positive for at least one of the seven DEC virulence genes investigated. The eaeA gene was the most isolated gene (29.44%) while the ipaH gene the least isolated (8.33%). The ipaH gene (p = 0.012) and the ST gene (stIa, p = 0.0001, and stIb, p = 0.019) were positively correlated with temperature. The detection of diarrhoeagenic E. coli virulence genes in the sediments of the Apies River shows that the sediments of this river might not only be a reservoir of faecal indicator bacteria like E. coli but also pathogenic strains of this bacterium. These organisms could represent a public health risk for poor communities relying on this water source for various purposes such as drinking and recreational use. There is therefore an urgent need to monitor these DEC pathotypes especially in areas without adequate water supplies. en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Worklist;18686
dc.subject Diarrhoeagenic E. coli en_US
dc.subject DEC en_US
dc.subject Environmental monitoring en_US
dc.subject Public health risk en_US
dc.subject Riverbed sediments en_US
dc.subject Rural communities en_US
dc.subject Virulence genes en_US
dc.subject Water quality en_US
dc.title Occurrence of diarrhoeagenic Escherichia coli virulence genes in water and bed sediments of a river used by communities in Gauteng, South Africa en_US
dc.type Article en_US


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