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dc.contributor.author Finnegan, T
dc.contributor.author Steenkamp, Paul A
dc.contributor.author Piater, LA
dc.contributor.author Dubery, IA
dc.date.accessioned 2017-05-15T11:57:27Z
dc.date.available 2017-05-15T11:57:27Z
dc.date.issued 2016-09
dc.identifier.citation Steenkamp P.A., Piater, L.A., Dubery, I.A. 2016.The Lipopolysaccharide-induced metabolome signature in Arabidopsis thaliana reveals dynamic reprogramming of Phytoalexin and Phytoanticipin pathways. In: PLoS ONE Vol. 11(9): e0163572. https://doi.org/10.1371/journal.pone.0163572 en_US
dc.identifier.issn 1932-6203
dc.identifier.uri http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0163572
dc.identifier.uri https://doi.org/10.1371/journal.pone.0163572
dc.identifier.uri http://hdl.handle.net/10204/9022
dc.description Copyright: © 2016 Finnegan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_US
dc.description.abstract Lipopolysaccharides (LPSs), as MAMP molecules, trigger the activation of signal transduction pathways involved in defence. Currently, plant metabolomics is providing new dimensions into understanding the intracellular adaptive responses to external stimuli. The effect of LPS on the metabolomes of Arabidopsis thaliana cells and leaf tissue was investigated over a 24 h period. Cellular metabolites and those secreted into the medium were extracted with methanol and liquid chromatography coupled to mass spectrometry was used for quantitative and qualitative analyses. Multivariate statistical data analyses were used to extract interpretable information from the generated multidimensional LC-MS data. The results show that LPS perception triggered differential changes in the metabolomes of cells and leaves, leading to variation in the biosynthesis of specialised secondary metabolites. Time-dependent changes in metabolite profiles were observed and biomarkers associated with the LPS-induced response were tentatively identified. These include the phytohormones salicylic acid and jasmonic acid, and also the associated methyl esters and sugar conjugates. The induced defensive state resulted in increases in indoleÐand other glucosinolates, indole derivatives, camalexin as well as cinnamic acid derivatives and other phenylpropanoids. These annotated metabolites indicate dynamic reprogramming of metabolic pathways that are functionally related towards creating an enhanced defensive capacity. The results reveal new insights into the mode of action of LPS as an activator of plant innate immunity, broadens knowledge about the defence metabolite pathways involved in Arabidopsis responses to LPS, and identifies specialised metabolites of functional importance that can be employed to enhance immunity against pathogen infection. en_US
dc.description.sponsorship The South African National Research Foundation (NRF: www.nrf.ac.za) is thanked for grant support (number 95818) to IAD. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The Council for Scientific and Industrial Research (CSIR) and the University of Johannesburg (UJ) are thanked for fellowship support to TF. Fidele Tugizimana is thanked for assisting with data deposition. en_US
dc.language.iso en en_US
dc.publisher PLOS en_US
dc.relation.ispartofseries Worklist;18044
dc.rights CC0 1.0 Universal *
dc.rights.uri http://creativecommons.org/publicdomain/zero/1.0/ *
dc.subject Lipopolysaccharides en_US
dc.subject Plant metabolomics en_US
dc.subject Biomarkers en_US
dc.subject Cellular metabolites en_US
dc.title Lipopolysaccharide-induced metabolome signature in Arabidopsis thaliana reveals dynamic reprogramming of Phytoalexin and Phytoanticipin pathways en_US
dc.type Article en_US


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