• Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica 

  Bulani, S; Moleleki, L; Albertyn, J; Moleleki, J (BioMed Central, 2012-05)

  In this study, a novel rDNA based plasmid was developed for display of heterologous proteins on the cell surface of Yarrowia lipolytica using the C- terminal end of the glycosylphosphatidylinositol (GPI) anchored Y. ...
• PALM and STORM: unlocking live-cell super-resolution 

  Henriques, R; Griffiths, C; Rego, EH; Mhlanga, Musa (Wiley Blackwell, 2011-05)

  Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy ...
• Effect of expressing an anti-HIV lectin, Griffithsin, in different plant cellular compartments 

  Stark, T; Chakauya, E; Kabamba, A; Morris, L; Rybicki, E; Chikwamba, Rachel K (2010-08)

  Sub-Saharan Africa remains the region most heavily affected by HIV, and the majority of new infections from heterosexual contact are in women. Griffithsin (GRFT), a lectin isolated from the red algae Griffithsia, has shown ...
• Morphological variation of sorghum landraces from semi-arid areas of Zimbabwe 

  Mujaju, C; Chakauya, E (2008)

  Sorghum (Sorghum bicolor) is one of the most important cereal crops cultivated in, the semi-arid areas of Zimbabwe where persistent drought, high temperatures and poor edaphic conditions are endemic. Because of their ...
• Issues on global food security 

  Chikwamba, Rachel K (2006-09-29)

  The presentation outlines the global status of food security and implications for meeting the Millennium Development Goals targets, with particular focus on Sub-Saharan Africa; trends in hunger, food availability, food ...
• Elicitor and fusarium-induced expression of NPR-1 like genes in banana 

  Endah, R; Beyene, G; Kiggundu, A; Van den Berg, N; Schlüter, U; Kunert, K; Chikwamba, Rachel K (Elsevier, 2008-11)

  NPR1 is an essential positive regulator of salicylic acid-induced PR gene expression and systemic acquired resistance. Two novel full-length NPR1-like genes; MNPR1A and MNPR1B, were isolated by application of the PCR and ...
• Analysis of genetic relationships of pearl millet (Pennisetum glaucum L.) landraces from Zimbabwe, using microsatellites 

  Chakauya, E; Tongoona, P (Academic Journals inc., 2008)

  Pearl millet (Pennisetum glaucum L.) is the fifth most important cereal crop in Zimbabwe, with great potential in food security and biofuels. However, the rich pearl millet genetic diversity is either partially characterised ...
• Towards engineering increased pantothenate (Vitamin B5) levels in plants 

  Chakauya, E; Coxon, KM; Wei, M; MacDonald, MV; Barsby, T; Abell, C; Smith, AG (Springer Verlag, 2008-11)

  Pantothenate (vitamin B5) is the precursor of the 4'-phosphopantetheine moiety of coenzyme A and acyl-carrier protein. It is made by plants and microorganisms de novo, but is a dietary requirement for animals. The pantothenate ...
• Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds 

  Brady, D; Dube, N; Petersen, RL (Academy of Science of South Africa, 2006-09)

  The application of highly substrate-specific catalysts, such as biocatalysts, can reduce the number of synthetic steps required to generate organic compounds. A wide range of bacteria and yeast cultures were enriched on ...
• Biological control agent against verticillium wilt 

  Gumede, WHN (2006-04)

  The biological control agent against verticillium wilt was presented at the SASM conference
• Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein 

  Ramchuran, SO; Berger, E; Du Plessis, E; Crampton, BG; Louw, ME (BioMed Central, 2006-09)

  In order to investigate the possibility of using the FliC display system for biotransformation in B. halodurans, construct pSECNLipC habouring the LipA gene from Geobacillus thermoleovorans was designed and evaluated in ...
• Novel platforms for essential biomolecules: expression of antibodies in plants 

  Chikwamba, Rachel K; Lotter, T; Ramesaar, K (2006-02-28)

  The demand for health products such as protein therapeutics and sub-unit vaccines to control infectious diseases far outweighs the current global production capacity of these molecules. These products are currently being ...
• Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds 

  Brady, D; Dube, N; Petersen, R (2006-02-28)

  Nitrile substrate profiles were determined for a wide range of microorganisms. Isolated bacteria and yeast were enriched on nitriles as the sole nitrogen source. Aliphatic and aromatic nitriles were investigated, as well ...
• Expression of HIV-1 antigens in plants as potential subunit vaccines 

  Meyers, A; Chakauya, E; Shepard, E; Tanzer, FL; Lynch, A; Williamson, A; Rybicki, EP (BioMed Central, 2008-06-23)

  Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the development of cheap, ...
• Expression of Rabies antibodies in tobacco and maize 

  Zungu, N; Lotter, T; Dube, N; Chakauya, E; Lezar, S; Chikwamba, Rachel K (2008-01)

  Rabies, an important disease in Asia and Africa, is an acute viral disease of the central nervous system that affects humans and other mammals. Upon bites or contact with rabid animals patients are immediately immunized ...
• Encapsulating probiotics with an interpolymer complex in supercritical carbon dioxide 

  Moolman, FS; Labuschagne, Philip W; Thantsa, MS; Van der Merwe, TL; Rolfes, H; Cloete, TE (Acad Science South Africa A S S AF, 2006)

  Traditional encapsulation methods in fortified foods and drug delivery applications present difficulties for ‘actives’, such as probiotics, sensitive to exposure to water, solvents, heat or oxygen, where ‘active’ refers ...
• Riding the tide of biopharming in Africa: considerations for risk assessment 

  Chakauya, E; Chikwamba, Rachel K; Rybick, EP (Acad Science South Africa, 2006-08)

  This article provides a background to biopharming, highlighting basic considerations for risk assessment and regulation in developing countries, with an emphasis on plant-based vaccines production in South Africa.
• Conversion of homothallic yeast to heterothallism through to gene disruption 

  Van Zyl, WH; Lodolo, EJ; Gericke, M (Springer-Verlag, 1993-04)

  A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho=neo disrupted allele was constructed by cloning a ...
• Bakers yeast-mediated transformations of alpha-keto epoxides 

  Meth-Cohn, O; Horak, RM; Fouché, Gerda (Royal Society Chemistry, 1994-06-07)

  Alpha beta-Epoxy ketones on treatment with baker's yeast yield different types of products depending on their substitution. Small groups such as H or Me attached at the epoxy end protect that end from attack. Thus, 1-acyl ...
• Role of alpha-glucosidase in the fermentable sugar composition of sorghum malt mashes 

  Taylor, JRN; Dewar, J (Institute of Brewing, 1994-11)

  The cause of the high glucose to maltose ratio in sorghum malt worts was studied. Mashing temperature and pH strongly affected both the amount of glucose and the proportion of glucose relative to total fermentable sugars. ...

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