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Show simple item record Ndlela, LL Schmidt, S 2016-12-05T09:39:15Z 2016-12-05T09:39:15Z 2016-02
dc.identifier.citation Ndlela, L.L. and Schmidt, S. 2016. Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms. SpringerPlus, 5(118), 1-9 en_US
dc.identifier.issn 2193-1801
dc.description Copyright: 2016 SpringerOpen en_US
dc.description.abstract This study assessed faecal matter from three indigenous South African herbivores-zebra, giraffe and impala-as a potential source for hydrolytically active aerobic and facultatively anaerobic bacteria. Herbivore droppings were collected freshly in a local nature reserve in Pietermaritzburg, South Africa. Soil samples adjacent to faecal collection sites and faeces from a domestic herbivore, the Nguni cow, were included as controls. Hydrolase and dehydrogenase activity in faecal matter and soil samples were measured by the fluorescein diacetate and the triphenyltetrazolium chloride assay. Viable counts and counts for amylase, cellulase, esterase and protease producers were established using plate count agar and solid media containing cellulose, skim milk, starch and Tween 80. Zebra droppings produced the highest hydrolase and dehydrogenase activity. Faecal matter of the three indigenous herbivores generally produced higher hydrolytic activity than Nguni cow faeces and soil controls, thereby confirming that these materials are potential targets for hydrolytic enzyme mining. en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Workflow;17413
dc.subject Faeces en_US
dc.subject Fluorescein diacetate en_US
dc.subject Giraffe en_US
dc.subject Hydrolases en_US
dc.subject Impala en_US
dc.subject Nguni en_US
dc.subject Triphenyltetrazolium chloride en_US
dc.subject Zebra en_US
dc.title Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms en_US
dc.type Article en_US

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