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dc.contributor.author Botes, AL
dc.contributor.author Lotter, J
dc.contributor.author Rhode, OHJ
dc.contributor.author Botha, A
dc.date.accessioned 2007-06-29T07:46:06Z
dc.date.available 2007-06-29T07:46:06Z
dc.date.issued 2005-01
dc.identifier.citation Botes, AL, et al. 2005. Interspecies differences in the enantioselectivity of epoxide hydrolases in Cryptococcus laurentii (Kufferath) C.E. Skinner and Cryptococcus podzolicus (Bab'jeva & Reshetova) Golubev. Systematic and Applied Microbiology, vol. 28(1), pp 27-33 en
dc.identifier.issn 0723-2020
dc.identifier.uri http://hdl.handle.net/10204/745
dc.description Copyright: 2005 Urban & Fischer Verlag en
dc.description.abstract Isolates representing Cryptococcus laurentii and Cryptococcus podzolicus, originating from soil of a heath land indigenous to South Africa, were screened for the presence of enantioselective epoxide hydrolases for 2, 2-disubstituted epoxides. Epoxide hydrolase activity for the 2, 2-disubstituted epoxide (+/-)-2-methyl-2-pentyl oxirane was found to be abundantly present in all isolates. The stereochemistry of the products formed by the epoxide hydrolase enzymes from isolates belonging to the two species (11 isolates representing C laurentii and 23 isolates representing C podzolicus) was investigated. The enantio preferences of the epoxide hydrolases for 2, 2-disubstituted epoxides of these two species were found to be opposite. All strains of C laurentii preferentially hydrolysed the (S)-epoxides while all C podzolicus isolates preferentially hydrolysed the (R)-epoxides of (+/-)-2, 2-disubstituted epoxides. These findings indicate that the stereochemistry of the products formed from 2, 2-disubstituted epoxides by the epoxide hydrolase enzymes of these yeasts should be evaluated as additional taxonomic criterion within the genus Cryptococcus. Also, the selectivity of some epoxide hydrolases originating from isolates of C podzolicus was high enough to be considered for application in biotransformations for the synthesis of enantiopure epoxides and vicinal diols. en
dc.language.iso en en
dc.publisher Urban & Fischer Verlag en
dc.subject Cryptococcus laurentii en
dc.subject Cryptococcus podzolicus en
dc.subject Epoxides en
dc.subject Epoxide hydrolases en
dc.title Interspecies differences in the enantioselectivity of epoxide hydrolases in Cryptococcus laurentii (Kufferath) C.E. Skinner and Cryptococcus podzolicus (Bab'jeva & Reshetova) Golubev en
dc.type Article en


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