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dc.contributor.author Yang, Y
dc.contributor.author Mkhonto, D
dc.contributor.author Cui, Q
dc.contributor.author Sahai, N
dc.date.accessioned 2012-05-08T14:16:56Z
dc.date.available 2012-05-08T14:16:56Z
dc.date.issued 2011-05
dc.identifier.citation Yang, Y, Mkhonto, D, Cui, Q and Sahai, N. 2011. Theoretical study of bone sialoprotein in bone biomineralization. Cells Tissues Organs, vol. 194(2-4), pp 182-187 en_US
dc.identifier.issn 1422-6405
dc.identifier.uri http://content.karger.com/ProdukteDB/produkte.asp?Doi=324648
dc.identifier.uri http://hdl.handle.net/10204/5848
dc.description Copyright: 2011 Karger. This is an ABSTRACT ONLY. en_US
dc.description.abstract Bone sialoprotein (BSP) is an acidic, non-collagenous protein specific to bone proposed previously to promote hydroxyapatite (HAP) nucleation and modulate HAP nanocrystal growth. Specifically, two phosphorylated acidic amino acid sequences in BSP, highly conserved across several vertebrates, are the proposed active sites. We selected one of these sites, i.e. (Sp) 2 E 8 , where Sp represents a phosphoserine as a model peptide to study the role of BSP. We used molecular dynamics simulations to determine whether an -helix or a random coil peptide conformation promotes templated HAP nucleation. A bioinformatics method helps infer preferential crystal growth directions by predicting the likely peptide conformations adsorbed on the (001), (100), and (110) crystal faces of HAP. Results suggest that, independent of conformation, no stable nucleating template is formed and, thus, the ion distributions in the vicinity of the peptide that eventually lead to a stable nucleus start out with disordered arrangements of ions. When adsorbed on all three faces, the Sp residues bind strongly regardless of the peptide conformation, and the Glu residues show different propensities to form helical conformations. The lack of geometrical templating between the peptide residues and all HAP surface sites indicates that adsorption and subsequent crystal growth modulation may be structurally nonspecific. en_US
dc.language.iso en en_US
dc.publisher Karger en_US
dc.relation.ispartofseries Workflow;8671
dc.subject Bone sialoprotein en_US
dc.subject Biomineralization en_US
dc.subject Nucleation en_US
dc.subject Hydroxyapatite en_US
dc.subject Computational simulation en_US
dc.title Theoretical study of bone sialoprotein in bone biomineralization en_US
dc.type Article en_US


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