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Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation

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dc.contributor.author Mthunzi, P
dc.contributor.author Dholakia, K
dc.contributor.author Gunn-Moore, F
dc.date.accessioned 2011-09-30T09:55:20Z
dc.date.available 2011-09-30T09:55:20Z
dc.date.issued 2010-07
dc.identifier.citation Mthunzi, P, Dholakia, K and Gunn-Moore, F. 2010. Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation. Journal of Biomedical Optics, Vol 15(4), pp 041507 en_US
dc.identifier.issn 1083-3668
dc.identifier.uri http://hdl.handle.net/10204/5189
dc.description Copright: 2010 Society of Photo-optical Instrumentation Engineers (SPIE) en_US
dc.description.abstract Recently, femtosecond laser pulses have been utilized for the targeted introduction of genetic matter into mammalian cells. This rapidly expanding and developing novel optical technique using a tightly focused laser light beam is called phototransfection. Extending previous studies, we show that femtosecond lasers can be used to phototransfect a range of different cell lines, and specifically that this novel technology can also transfect mouse embryonic stem cell colonies with 25% efficiency. Notably, we show the ability of differentiating these cells into the extraembryonic endoderm using phototransfection. Furthermore, we present two new findings aimed at optimizing the phototransfection method and improving applicability: first, the influence of the cell passage number on the transfection efficiency is explored and, second, the ability to enhance the transfection efficiency via whole culture treatments. Our results should encourage wider uptake of this methodology en_US
dc.language.iso en en_US
dc.publisher Society of Photo-optical Instrumentation Engineers (SPIE) en_US
dc.relation.ispartofseries Workflow request;6084
dc.subject Phototransfection en_US
dc.subject Femtosecond en_US
dc.subject Laser pulses en_US
dc.subject Cell arresting en_US
dc.subject Neuroblastomas en_US
dc.subject Pluripotent stem cells en_US
dc.subject Embryonic stem cell differentiation en_US
dc.subject Biomedical optics en_US
dc.subject Mammalian cells en_US
dc.title Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation en_US
dc.type Article en_US


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