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dc.contributor.author Malabi, Rudzani
dc.contributor.author Manoto, Sello L
dc.contributor.author Ombinda-Lemboumba, Saturin
dc.contributor.author Maaza, Malik
dc.contributor.author Mthunzi-Kufa, Patience
dc.date.accessioned 2019-08-26T07:20:49Z
dc.date.available 2019-08-26T07:20:49Z
dc.date.issued 2019-05
dc.identifier.citation Malabi, R., Manoto, S.L., Ombinda‐Lemboumba, S., Maaza, M. & Mthunzi‐Kufa, P. 2019. Laser‐enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus‐1 infected TZMbl cells. Journal of Biophotonics, pp. 1-9. https://doi.org/10.1002/jbio.201800424 en_US
dc.identifier.issn 1864-063X
dc.identifier.issn 1864-0648
dc.identifier.uri https://doi.org/10.1002/jbio.201800424
dc.identifier.uri https://www.ncbi.nlm.nih.gov/pubmed/31140728
dc.identifier.uri https://onlinelibrary.wiley.com/doi/full/10.1002/jbio.201800424
dc.identifier.uri http://hdl.handle.net/10204/11098
dc.description Copyright: 2019 Wiley Online Library: Due to copyright restrictions, the attached PDF file only contains the abstract version of the full-text item. For access to the full-text item, please consult the publisher's website. The definitive version of the work is published Journal of Biophotonics, pp. 1-9. https://doi.org/10.1002/jbio.201800424 en_US
dc.description.abstract The introduction of highly active antiretroviral therapy (HAART) has significantly increased life expectancy and improved management of the human immunodeficiency virus-1 (HIV-1) disease globally. This well-established treatment regime has shown to reduce viral capacity to undetectable limits when using traditional clinical assays. The establishment of viral reservoirs during the early stages of infection are the major contributors to failure of the current regimens to eradicate HIV-1 infection since the reservoirs are not affected by antiretroviral drugs (ARVs). Therefore, advanced modification of the present treatment and investigation of novel antiretroviral drug delivery system are needed. The aim of this study was to use femtosecond (fs) laser pulses to deliver ARVs into HIV-1 infected TZMbl cells. Different ARVs were translocated into TZMbl cells using fs pulsed laser (800 nm) with optimum power of 4 µW and 10 ms laser to cell exposure time. Changes in cellular processes were evaluated using cellular morphology, viability, cytotoxicity and luciferase activity assays. Cells treated with the laser in the presence of ARVs showed a significant reduction in viral infectivity, cell viability and an increase in cytotoxicity. This study demonstrated that fs laser pulses were highly effective in delivering ARVs into HIV-1 infected TZMbl cells, causing a significant reduction in HIV-1 infection. en_US
dc.language.iso en en_US
dc.publisher Wiley Online Library en_US
dc.relation.ispartofseries Workflow;22582
dc.subject Antiretroviral drugs en_US
dc.subject Femtosecond laser pulses en_US
dc.subject HIV-1 en_US
dc.subject Optical drug delivery en_US
dc.subject Photo-translocation en_US
dc.subject TZMbl cells en_US
dc.title Laser-enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus-1 infected TZMbl cells en_US
dc.type Article en_US


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