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dc.contributor.author Malabi, Rudzani
dc.contributor.author Lugongolo, Masixole Y
dc.contributor.author Thobakgale, Setumo L
dc.contributor.author Manoto, Sello L
dc.contributor.author Mthunzi-Kufa, Patience
dc.contributor.author Ombinda-Lemboumba, Saturnin
dc.date.accessioned 2019-08-14T06:59:02Z
dc.date.available 2019-08-14T06:59:02Z
dc.date.issued 2019-02
dc.identifier.citation Ombinda-Lemboumba, S., Malabi, R., Lugongolo, M.Y., Thobakgale, S.L., Manoto, S.L. and Mthunzi-Kufa, P. 2019. Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement. Journal of Biophotonics, V12(7), pp 1-8. en_US
dc.identifier.issn 1864-0648
dc.identifier.uri https://onlinelibrary.wiley.com/doi/10.1002/jbio.201800349
dc.identifier.uri http://www.interscience.wiley.com/
dc.identifier.uri http://hdl.handle.net/10204/11081
dc.description Copyright: 2019 Wiley Interscience. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website at http://www.interscience.wiley.com. en_US
dc.description.abstract Transmission measurement has been perceived as a potential candidate for label-free investigation of biological material. It is a real-time, label-free and non-invasive optical detection technique that has found wide applications in pharmaceutical industry as well as the biological and medical fields. Combining transmission measurement with optical trapping has emerged as a powerful tool allowing stable sample trapping, while also facilitating transmittance data analysis. In this study, a near-infrared laser beam emitting at a wavelength of 1064 nm was used for both optical trapping and transmission measurement investigation of human immunodeficiency virus 1 (HIV-1) infected and uninfected TZM-bl cells. The measurements of the transmittance intensity of individual cells in solution were carried out using a home built optical trapping system combined with laser transmission setup using a single beam gradient trap. Transmittance spectral intensity patterns revealed significant differences between the HIV-1 infected and uninfected cells. This result suggests that the transmittance data analysis technique used in this study has the potential to differentiate between infected and uninfected TZM-bl cells without the use of labels. The results obtained in this study could pave a way into developing an HIV-1 label-free diagnostic tool with possible applications at the point of c en_US
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.relation.ispartofseries Worklist;22576
dc.subject HIV-1 infection en_US
dc.subject Label-free detection en_US
dc.subject Mammalian cells en_US
dc.subject Optical rapping en_US
dc.subject Transmission measurement en_US
dc.title Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement en_US


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