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dc.contributor.author Griffith, Derek J
dc.contributor.author Bone, Emma L
dc.contributor.author Thomalla, Sandy J
dc.contributor.author Bernard, Stewart
dc.date.accessioned 2019-04-02T07:30:54Z
dc.date.available 2019-04-02T07:30:54Z
dc.date.issued 2018-07
dc.identifier.citation Griffith, D.J. et al. 2018. Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield. Optics Express, vol. 26(15): 18863-18877 en_US
dc.identifier.issn 1094-4087
dc.identifier.uri https://www.osapublishing.org/oe/abstract.cfm?uri=oe-26-15-18863&origin=search
dc.identifier.uri https://doi.org/10.1364/OE.26.018863
dc.identifier.uri http://hdl.handle.net/10204/10899
dc.description This is an open access article published in Optics Express, vol. 26(15): 18863-18877 en_US
dc.description.abstract A multi-excitation fluorometer (MFL, JFE Advantech Co., Ltd.), originally designed to discriminate between phytoplankton species present within a population, has been redirected for use in fluorescence quantum yield (FQY) determination. While this calibration for apparent FQY requires no modification of the MFL, it is necessary to have an independent measurement of the spectral absorption coefficient of the subject fluid. Two different approaches to calibration were implemented. The primary method made use of reference fluorescent dye solutions of known quantum yield. The second method made use of acrylic fluorescent plaques and films. The two methods yielded consistent results, except in the 570 and 590 nm LED channels of the MFL. Application of the MFL in FQY determination is illustrated with an in situ Southern Ocean sample. en_US
dc.language.iso en en_US
dc.publisher OSA Publishing en_US
dc.relation.ispartofseries Worklist;22312
dc.relation.ispartofseries Worklist;22289
dc.subject Multi-excitation fluorometer en_US
dc.subject MFL en_US
dc.subject Phytoplankton species en_US
dc.title Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield en_US
dc.type Article en_US


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