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dc.contributor.author Maphanga, Charles P
dc.contributor.author Ombinda-Lemboumba, Saturnin
dc.contributor.author Manoto, Sello L
dc.contributor.author Maaza, Malik
dc.contributor.author Mthunzi-Kufa, Patience
dc.date.accessioned 2017-09-19T08:31:41Z
dc.date.available 2017-09-19T08:31:41Z
dc.date.issued 2017-01
dc.identifier.citation Maphanga, C.P., Ombinda-Lemboumba, S., Manoto, S.L., Maaza, M. and Mthunzi-Kufa, P. 2017. Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies. In: Proceedings of SPIE 10062, Optical Interactions with Tissue and Cells XXVIIISan Francisco, California, USA, 26 January - 03 February 2017 en_US
dc.identifier.uri http://spie.org/Publications/Proceedings/Paper/10.1117/12.2252291?origin_id=x4323&start_year=1963
dc.identifier.uri http://hdl.handle.net/10204/9581
dc.description Proceedings of SPIE 10062, Optical Interactions with Tissue and Cells XXVIIISan Francisco, California, USA, 26 January - 03 February 2017. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website en_US
dc.description.abstract Human immunodeficiency virus (HIV-1) infection still remains one amongst the world’s most challenging infections since its discovery. Antiretroviral therapy is the recommended treatment of choice for HIV-1 infection taken by patients orally. The highly active antiretroviral therapy (HAART) prevents the replication of HIV-1 and further destruction of the immune system, therefore enabling the body to fight opportunistic life-threatening infections, cancers, and also arrest HIV infection from advancing to AIDS. The major challenge with HAART is the inability to reach the viral reservoirs where the HIV-1 remains latent and persistent, leading to inability to fully eradicate the virus. This study is aimed at initially designing and assembling a fully functional optical translocation setup to optically deliver antiretroviral drugs into HIV-1 infected cells in a targeted manner using Gaussian beam mode femtosecond laser pulses in-vitro. The main objective of our study is to define the in-vitro drug photo-translocation parameters to allow future design of an efficient drug delivery device with potential in-vivo drug delivery applications. In our experiments, HEK 293T cells were used to produce HIV-1 enveloped pseudovirus (ZM53) to infect TZM-bl cells which were later treated with laser pulses emitted by a titanium sapphire laser (800 nm, 1KHz, 113 fs, ~ 6.5 µW) to create sub-microscopic pores on the cell membrane enabling influx of extracellular media. Following laser treatment, changes in cellular responses were analysed using cell morphology studies, cytotoxicity, and luciferase assay studies. Controls included laser untreated cells incubated with the drug for 72 hours. The data in this study was statistically analysed using the SigmaPlot software version 13. en_US
dc.language.iso en en_US
dc.publisher SPIE en_US
dc.relation.ispartofseries Workflow;18700
dc.subject Human Immunodeficiency Virus en_US
dc.subject HIV en_US
dc.subject Highly Active Antiretroviral Therapy en_US
dc.subject HAART en_US
dc.subject Acquired Immunodeficiency Syndrome en_US
dc.subject AIDS en_US
dc.subject Gaussian beams en_US
dc.subject Femtosecond pulses en_US
dc.subject Optical translocation en_US
dc.subject TZM-bl cells en_US
dc.subject Sub-microscopic pores en_US
dc.subject Cell morphology en_US
dc.subject Cell cytotoxicity en_US
dc.subject Targeted drug delivery en_US
dc.title Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [conference paper] en_US
dc.type Presentation en_US


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