• The effect of thyme oil low-density polyethylene impregnated pellets in polylactic acid sachets on storage quality of ready-to-eat avocado 

  Bill, M; Pillai, Sreejarani K; Tinyane, P; Sinha Ray, Suprakas; Sivakumar, D (Springer, 2018-01)

  After earlier promising results for the control of anthracnose in avocado fruit by thyme vapours, our studies were extended to commercial use in tray packs. The effect of thyme oil low-density polyethylene impregnated ...
• Phosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1 

  Muleya, V; Marondedze, C; Wheeler, JI; Thomas, L; Mok, YF; Griffin, MD; Manallack, DT; Kwezi, Lusisizwe; Lilley, KS; Gehring, C; Irving, HR (Portland Press, 2016-10)

  Phytosulfokines (PSKs) are plant peptide hormones that co-regulate plant growth, differentiation and defense responses. PSKs signal through a plasma membrane localized leucine-rich repeat receptor-like kinase (phytosulfokine ...
• The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling 

  Wheeler, J; Wong, A; Marondedze, C; Groen, AJ; Kwezi, Lusisizwe (Wiley, 2017-06)

  The brassinosteroid receptor brassinosteroid insensitive 1 (BRI1) is a member of the leucine-rich repeat receptor-like kinase family. The intracellular kinase domain of BRI1 is an active kinase and also encapsulates a ...
• Efficient in vitro and in vivo activity of glyco-engineered plant-produced rabies monoclonal antibodies E559 and 62-71-3 

  Tsekoa, Tsepo L; Lotter-Stark, Therese; Buthelezi, Sindisiwe; Chakauya, Ereck; Stoychev, Stoyan H; Chikwamba, Rachel (PLoS ONE, 2016-07)

  Rabies is a neglected zoonotic disease that has no effective treatment after onset of illness. However the disease can be prevented effectively by prompt administration of post exposure prophylaxis which includes administration ...
• The African perspective I: Using the new biosciences to support the African development agenda 

  Morris, EJ; Erasmus, C; O'Kennedy, M (Taylor & Francis Group publishers, 2016-09)

  Biopharmaceutical proteins and vaccines are traditionally produced in bacteria, eggs, yeast and animal cell cultures and are well established industries. More recently, these molecules are produced in plants and became ...
• Physiological responses of selected African sorghum landraces to progressive water stress and re-watering 

  Devnarain, N; Crampton, BG; Chikwamba, Rachel K; Becker, JVW; O'Kennedy, MM (Elsevier, 2016-03)

  Sorghum is particularly drought tolerant compared with other cereal crops and is favoured for subsistence farming in water scarce regions of the world. This study was conducted to identify South African sorghum landraces ...
• Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils 

  Ramond, JB; Lako, JDW; Stafford, WHL; Tuffin, MI; Cowan, DA (Wiley-VCH Verlag, 2015-02)

  Ammonia-oxidizing bacteria (AOB) are essential in the biogeochemical cycling of nitrogen as they catalyze the rate-limiting oxidation of ammonia into nitrite. Since their first isolation in the late 19th century, ...
• Single-dose monomeric HA subunit vaccine generates full protection from influenza challenge 

  Mallajosyula, JK; Hiatt, E; Hume, S; Johnson, A; Jeevan, T; Chikwamba, Rachel K; Pogue, GP; Bratcher; Haydon, H; Webby, RJ; McCormick, AA (Taylor & Francis, 2014-03)

  Recombinant subunit vaccines are an efficient strategy to meet the demands of a possible influenza pandemic, because of rapid and scalable production. However, vaccines made from recombinant hemagglutinin (HA) subunit ...
• Seasonal variations in nutrients and secondary metabolites in semi-arid savannas depend on year and species 

  Scogings, PF; Hattas, D; Skarpe, C; Hjältén, J; Dziba, L; Zobolo, A; Rooke, T (Elsevier, 2015-03)

  Semi-arid savannas are characterised by alternating wet and dry seasons and large inter-annual rainfall fluctuations that affect plant growth. Carbon-based secondary metabolites (CBSMs) vary inversely with growth and ...
• The effect of temperature on pressurised hot water extraction of pharmacologically important metabolites as analysed by UPLC-qTOF-MS and PCA 

  Khoza, BS; Chimuka, L; Mukwevho, E; Steenkamp, PA; Madala, NE (Hindawi Publishing Corporation, 2014-10)

  Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new extraction methods are crucial. In the current study, an organic ...
• Genomic Resources 

  Barros, E; Morris, EJ (CRC Press, 2014)

  The fastest growing use of maize is for the production of fuel ethanol using the enzymatic conversion of corn starch to glucose and then to ethanol as well by converting the cellulosic (non-food) parts of maize to ethanol. ...
• Co-suppression of synthesis of major x-kafirin sub-class together with y-kafirin-1 and y-kafirin-2 required for substantially improved protein digestibility in transgenic sorghum 

  Grootboom, AW; Mkhonza, NL; Mbambo, Z; O'Kennedy, MM; Da Silva, LS; Taylor, J; Taylor, JRN; Chikwamba, Rachel K; Mehlo, L (Springer link, 2014-01)

  Co-suppressing major kafirin sub-classes is fundamental to improved protein digestibility and nutritional value of sorghum. The improvement is linked to an irregularly invaginated phenotype of protein bodies.
• Value added products from microalgae 

  Naidoo, T; Zulu, N; Maharajh, Dheepak M; Lalloo, R (CRC Press, 2013-09)

  Microalgae represent a bio-diverse resource (Metting, 1996; Pulz and Gross, 2004). The complexity of their chemical composition and range of biochemical products make these organisms exploitable resources for valuable and ...
• Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells 

  Tugizimana, F; Steenkamp, PA; Piater, LA; Dubery, IA (Elsevier, 2014-01)

  Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used to investigate the effect of ...
• Metabolomic analysis of isonitrosoacetophenone-induced perturbations in phenolic metabolism of Nicotiana tabacum cells 

  Madala, NE; Steenkamp, PA; Piater, LA; Dubery, IA (Elsevier, 2013-10)

  Plants have developed biochemical and molecular responses to adapt to different stress environments. One of the characteristics of the multi-component defence response is the production of defence-related metabolites. Plant ...
• Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum 

  Mehlo, L; Mbambo, Z; Bado, S; Lin, J; Moagi, SM; Buthelezi, S; Stoychev, S; Chikwamba, Rachel K (Elsevier, 2013-09)

  Physical and biochemical analysis of protein polymorphisms in seed storage proteins of a mutant population of sorghum revealed a mutant with redirected accumulation of kafirin proteins in the germ. The change in storage ...
• Biolistic-mediated transformation protocols for maize and pearl millet using pre-cultured immature zygotic embryos and embryogenic tissue 

  O'Kennedy, MM; Stark, HC; Dube, N (Springer, 2011-01)

  Maize (Zea mays L.) is the most important cereal food crop in sub-Saharan Africa and Latin America, and a key feed crop in Asia, whereas pearl millet [Pennisetum glaucum (L.) R. Br.] is a staple food that supplies a major ...
• Identification of mycotoxigenic fungi using an oligonucleotide microarray 

  Barros, E (Springer, 2013)

  Mycotoxins are secondary metabolites produced by fungi; they can play a role as food contaminants and have the ability to negatively influence human and animal health. To improve food safety and to protect consumers from ...
• The short and long of it: shorter chromatographic analysis suffice for sample classification during UHPLC-MS-based metabolic fingerprinting 

  Madala, NE; Tugizimana, F; Steenkamp, PA; Piater, LA; Dubery, IA (Springer-Verlag, 2013-03)

  Ultra high-performance liquid chromatography hyphenated to mass spectrometry (UHPLC-MS) technologies has been widely applied in metabolomics, and the high resolution and peak capacity thereof are only some of the key aspects ...
• Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions 

  Madala, NE; Steenkamp, PA; Piater, LA; Dubery, IA (Springer Netherlands, 2012-07)

  Nicotiana tabacum cell suspensions, 2g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing 40-hexopyranosyloxy-30-met ...

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