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Show simple item record Bain, RES Woodall, C Elliott, J Arnold, BF Tung, R Morley, R du Preez, M Bartram, JK Davis, AP Gundry, SW Pedley, S 2016-05-16T10:21:47Z 2016-05-16T10:21:47Z 2015-10
dc.identifier.citation Bain, RES, Woodall, C, Elliott, J, Arnold, BF, Tung, R, Morley, R, du Preez, M, Bartram, JK, Davis, AP, Gundry, SW and Pedley, S. 2015. Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters. PLoS One, pp 1-13 en_US
dc.identifier.issn 1932-6203
dc.description Copyright: 2015 PLoS One en_US
dc.description.abstract The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in lowincome countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti- Tray1. AT was compared with IDEXX Colilert-181 and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-181 for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely. en_US
dc.language.iso en en_US
dc.publisher PLOS ONE en_US
dc.relation.ispartofseries Workflow;15926
dc.subject Escherichia coli en_US
dc.subject Filtration en_US
dc.subject Sewage effluent en_US
dc.subject Sub-tropical study en_US
dc.subject Surface water en_US
dc.subject Water Quality en_US
dc.title Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters en_US
dc.type Article en_US

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