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Conversion of homothallic yeast to heterothallism through to gene disruption

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dc.contributor.author Van Zyl, WH
dc.contributor.author Lodolo, EJ
dc.contributor.author Gericke, M
dc.date.accessioned 2007-07-02T07:06:44Z
dc.date.available 2007-07-02T07:06:44Z
dc.date.issued 1993-04
dc.identifier.citation Van Zyl, WH, Lodolo, EJ and Gericke, M. 1993. Conversion of homothallic yeast to heterothallism through to gene disruption. Current Genetics, vol. 23(4), pp 290-294 en
dc.identifier.issn 0172-8083
dc.identifier.uri http://hdl.handle.net/10204/831
dc.description Copyright: 1993 Springer-Verlag en
dc.description.abstract A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho=neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothallic diploid S. cerevisiae strain with plasmid DNA containing the ho=neo allele yielded G418-resistant yeast transformants in which one of the HO alleles was replaced by the disrupted ho=neo allele. Meiotic tetrad analysis of four-spored asci from these G418-resistant transformants gave rise to haploid heterothallic and diploid homothallic tetrad progeny. The presence of the ho=neo and HO alleles in the heterothallic and homothallic progeny was confirmed by Southern-blot analysis. en
dc.language.iso en en
dc.publisher Springer-Verlag en
dc.subject HO gene disruptions en
dc.subject G418 resistance en
dc.subject Saccharomyces cerevisiae en
dc.subject Homothallism en
dc.title Conversion of homothallic yeast to heterothallism through to gene disruption en
dc.type Article en
dc.identifier.apacitation Van Zyl, W., Lodolo, E., & Gericke, M. (1993). Conversion of homothallic yeast to heterothallism through to gene disruption. http://hdl.handle.net/10204/831 en_ZA
dc.identifier.chicagocitation Van Zyl, WH, EJ Lodolo, and M Gericke "Conversion of homothallic yeast to heterothallism through to gene disruption." (1993) http://hdl.handle.net/10204/831 en_ZA
dc.identifier.vancouvercitation Van Zyl W, Lodolo E, Gericke M. Conversion of homothallic yeast to heterothallism through to gene disruption. 1993; http://hdl.handle.net/10204/831. en_ZA
dc.identifier.ris TY - Article AU - Van Zyl, WH AU - Lodolo, EJ AU - Gericke, M AB - A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho=neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothallic diploid S. cerevisiae strain with plasmid DNA containing the ho=neo allele yielded G418-resistant yeast transformants in which one of the HO alleles was replaced by the disrupted ho=neo allele. Meiotic tetrad analysis of four-spored asci from these G418-resistant transformants gave rise to haploid heterothallic and diploid homothallic tetrad progeny. The presence of the ho=neo and HO alleles in the heterothallic and homothallic progeny was confirmed by Southern-blot analysis. DA - 1993-04 DB - ResearchSpace DP - CSIR KW - HO gene disruptions KW - G418 resistance KW - Saccharomyces cerevisiae KW - Homothallism LK - https://researchspace.csir.co.za PY - 1993 SM - 0172-8083 T1 - Conversion of homothallic yeast to heterothallism through to gene disruption TI - Conversion of homothallic yeast to heterothallism through to gene disruption UR - http://hdl.handle.net/10204/831 ER - en_ZA


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