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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/825

Title: Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish
Authors: Lalloo, R
Ramchuran, SO
Ramduth, DM
Gorgens, J
Keywords: Aquaculture
Bacillus spp
Bacterial pathogens
Biological agent
Water quality
Mud sediment
Cyprinus carpio
Issue Date: Feb-2007
Publisher: Blackwell Publishing
Citation: Lalloo, R et al. 2007. Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish. Journal of Applied Microbiology, pp 1-9
Abstract: Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibi-tion of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a pre-liminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products.
Description: Copyright: 2007 Blackwell Publishing
URI: http://hdl.handle.net/10204/825
ISSN: 1364-5072
Appears in Collections:Bioprocessing development
General science, engineering & technology

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