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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/812

Title: Stable transformation of Hi-II maize using the particle inflow gun
Authors: O'Kennedy, MM
Burger, JT
Watson, TG
Keywords: Embryogenic type II callus
Transgenic plants
Immature embryos
Bombardment
Genetic engineering
Issue Date: Apr-1998
Publisher: Bureau Scientific Publ
Citation: O'Kennedy, MM, Burger, JT and Watson, TG. 1998. Stable transformation of Hi-II maize using the particle inflow gun. South African Journal of Science, Vol. South African Journal of Science 94(4), pp 188-192
Abstract: Focuses on the transformation of the embryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, by microprojectile bombardment using a particle inflow gun. Use of the plasmid pAHC25; Tissue culture and plant growth conditions; Optimization of bombardment conditions by transient expression; Southern blot analysis.
Description: Copyright: 1998 Bureau Scientific Publ
URI: http://hdl.handle.net/10204/812
ISSN: 0038-2353
Appears in Collections:Structural biology
General science, engineering & technology

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