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http://hdl.handle.net/10204/812
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| Title: | Stable transformation of Hi-II maize using the particle inflow gun |
| Authors: | O'Kennedy, MM
Burger, JT
Watson, TG |
| Keywords: | Embryogenic type II callus
Transgenic plants
Immature embryos
Bombardment
Genetic engineering |
| Issue Date: | Apr-1998 |
| Publisher: | Bureau Scientific Publ |
| Citation: | O'Kennedy, MM, Burger, JT and Watson, TG. 1998. Stable transformation of Hi-II maize using the particle inflow gun. South African Journal of Science, Vol. South African Journal of Science 94(4), pp 188-192 |
| Abstract: | Focuses on the transformation of the embryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, by microprojectile bombardment using a particle inflow gun. Use of the plasmid pAHC25; Tissue culture and plant growth conditions; Optimization of bombardment conditions by transient expression; Southern blot analysis. |
| Description: | Copyright: 1998 Bureau Scientific Publ |
| URI: | http://hdl.handle.net/10204/812 |
| ISSN: | 0038-2353 |
| Appears in Collections: | Structural biology
General science, engineering & technology
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