dc.contributor.author |
De Souza Gil, E
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dc.contributor.author |
Rezende, SG
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dc.contributor.author |
Ribeiro Júnior, EJM
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dc.contributor.author |
Barcelos, HT
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dc.contributor.author |
Scalize, PS
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dc.contributor.author |
Santiago, MF
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dc.contributor.author |
Quintino, MP
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dc.contributor.author |
Somerset, VS
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dc.date.accessioned |
2015-03-12T10:07:53Z |
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dc.date.available |
2015-03-12T10:07:53Z |
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dc.date.issued |
2014 |
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dc.identifier.citation |
De Souza Gil, E, Rezende, S.G, Ribeiro Júnior, E.J.M, Barcelos, H.T, Scalize, P.S, Santiago, M.F, Quintino, M.P and Somerset, V.S. 2014. Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters. In: Sensing in Electroanalysis, University Press Centre, Pardubice, Czech Republic, vol.8, pp 227-240 |
en_US |
dc.identifier.isbn |
978-80-7395-782-7 |
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dc.identifier.uri |
http://dspace.upce.cz/bitstream/10195/58400/1/SouzaGilE_DevelopmentOfLaccase_2014.pdf
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dc.identifier.uri |
http://hdl.handle.net/10204/7936
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dc.description |
Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. |
en_US |
dc.description.abstract |
Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV (sups-1) and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L(sup-1), with a detection limit of 30 nmol L(sup-1). This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
University Press Centre |
en_US |
dc.relation.ispartofseries |
Workflow;14109 |
|
dc.subject |
Biosensor |
en_US |
dc.subject |
Laccase |
en_US |
dc.subject |
Phenolic micropollutants |
en_US |
dc.subject |
Catechol |
en_US |
dc.subject |
Pycnoporus sanguineus fungi |
en_US |
dc.subject |
Functionalised silica |
en_US |
dc.title |
Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters |
en_US |
dc.type |
Book Chapter |
en_US |
dc.identifier.apacitation |
De Souza Gil, E., Rezende, S., Ribeiro Júnior, E., Barcelos, H., Scalize, P., Santiago, M., ... Somerset, V. (2014). Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters., <i>Workflow;14109</i> University Press Centre. http://hdl.handle.net/10204/7936 |
en_ZA |
dc.identifier.chicagocitation |
De Souza Gil, E, SG Rezende, EJM Ribeiro Júnior, HT Barcelos, PS Scalize, MF Santiago, MP Quintino, and VS Somerset. "Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters" In <i>WORKFLOW;14109</i>, n.p.: University Press Centre. 2014. http://hdl.handle.net/10204/7936. |
en_ZA |
dc.identifier.vancouvercitation |
De Souza Gil E, Rezende S, Ribeiro Júnior E, Barcelos H, Scalize P, Santiago M, et al. Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters.. Workflow;14109. [place unknown]: University Press Centre; 2014. [cited yyyy month dd]. http://hdl.handle.net/10204/7936. |
en_ZA |
dc.identifier.ris |
TY - Book Chapter
AU - De Souza Gil, E
AU - Rezende, SG
AU - Ribeiro Júnior, EJM
AU - Barcelos, HT
AU - Scalize, PS
AU - Santiago, MF
AU - Quintino, MP
AU - Somerset, VS
AB - Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV (sups-1) and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L(sup-1), with a detection limit of 30 nmol L(sup-1). This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol.
DA - 2014
DB - ResearchSpace
DP - CSIR
KW - Biosensor
KW - Laccase
KW - Phenolic micropollutants
KW - Catechol
KW - Pycnoporus sanguineus fungi
KW - Functionalised silica
LK - https://researchspace.csir.co.za
PY - 2014
SM - 978-80-7395-782-7
T1 - Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters
TI - Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters
UR - http://hdl.handle.net/10204/7936
ER -
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en_ZA |