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dc.contributor.author De Souza Gil, E
dc.contributor.author Rezende, SG
dc.contributor.author Ribeiro Júnior, EJM
dc.contributor.author Barcelos, HT
dc.contributor.author Scalize, PS
dc.contributor.author Santiago, MF
dc.contributor.author Quintino, MP
dc.contributor.author Somerset, VS
dc.date.accessioned 2015-03-12T10:07:53Z
dc.date.available 2015-03-12T10:07:53Z
dc.date.issued 2014
dc.identifier.citation De Souza Gil, E, Rezende, S.G, Ribeiro Júnior, E.J.M, Barcelos, H.T, Scalize, P.S, Santiago, M.F, Quintino, M.P and Somerset, V.S. 2014. Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters. In: Sensing in Electroanalysis, University Press Centre, Pardubice, Czech Republic, vol.8, pp 227-240 en_US
dc.identifier.isbn 978-80-7395-782-7
dc.identifier.uri http://dspace.upce.cz/bitstream/10195/58400/1/SouzaGilE_DevelopmentOfLaccase_2014.pdf
dc.identifier.uri http://hdl.handle.net/10204/7936
dc.description Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. en_US
dc.description.abstract Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV (sups-1) and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L(sup-1), with a detection limit of 30 nmol L(sup-1). This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol. en_US
dc.language.iso en en_US
dc.publisher University Press Centre en_US
dc.relation.ispartofseries Workflow;14109
dc.subject Biosensor en_US
dc.subject Laccase en_US
dc.subject Phenolic micropollutants en_US
dc.subject Catechol en_US
dc.subject Pycnoporus sanguineus fungi en_US
dc.subject Functionalised silica en_US
dc.title Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters en_US
dc.type Book chapter en_US


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