dc.contributor.author |
Lakay, FM
|
|
dc.contributor.author |
Botha, A
|
|
dc.contributor.author |
Prior, BA
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|
dc.date.accessioned |
2007-06-29T07:56:00Z |
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dc.date.available |
2007-06-29T07:56:00Z |
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dc.date.issued |
2007-01 |
|
dc.identifier.citation |
Lakay FM, Botha A, Prior BA. 2007. Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils. Journal of Applied Microbiology, 102(1), pp 265-273 |
en |
dc.identifier.issn |
1364-5072 |
|
dc.identifier.uri |
http://hdl.handle.net/10204/756
|
|
dc.description |
http://www.ncbi.nlm.nih.gov/entrez/query. |
en |
dc.description.abstract |
Three different soil DNA isolation and four purification strategies were compared on different soil samples with variable rates of success. Bead beating extraction gave significantly higher DNA yields than microwave-based and liquid nitrogen grinding DNA extraction methods. The inclusion of soil washing prior to cell lysis decreased the amount of purification steps required. Although these soil types differed, polyvinylpolypyrrolidone (PVPP)-sepharose 2B column elution was sufficient for all three samples, yielding DNA pure enough for successful application in molecular studies. One soil sample retained 80% of the initial DNA after successful purification. |
en |
dc.language.iso |
en |
en |
dc.publisher |
Blackwell Publishing |
en |
dc.subject |
Soil DNA isolation |
en |
dc.subject |
Liquid nitrogen |
en |
dc.subject |
Humic acid |
en |
dc.subject |
Soil |
en |
dc.title |
Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils |
en |
dc.type |
Article |
en |
dc.identifier.apacitation |
Lakay, F., Botha, A., & Prior, B. (2007). Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils. http://hdl.handle.net/10204/756 |
en_ZA |
dc.identifier.chicagocitation |
Lakay, FM, A Botha, and BA Prior "Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils." (2007) http://hdl.handle.net/10204/756 |
en_ZA |
dc.identifier.vancouvercitation |
Lakay F, Botha A, Prior B. Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils. 2007; http://hdl.handle.net/10204/756. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Lakay, FM
AU - Botha, A
AU - Prior, BA
AB - Three different soil DNA isolation and four purification strategies were compared on different soil samples with variable rates of success. Bead beating extraction gave significantly higher DNA yields than microwave-based and liquid nitrogen grinding DNA extraction methods. The inclusion of soil washing prior to cell lysis decreased the amount of purification steps required. Although these soil types differed, polyvinylpolypyrrolidone (PVPP)-sepharose 2B column elution was sufficient for all three samples, yielding DNA pure enough for successful application in molecular studies. One soil sample retained 80% of the initial DNA after successful purification.
DA - 2007-01
DB - ResearchSpace
DP - CSIR
KW - Soil DNA isolation
KW - Liquid nitrogen
KW - Humic acid
KW - Soil
LK - https://researchspace.csir.co.za
PY - 2007
SM - 1364-5072
T1 - Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils
TI - Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils
UR - http://hdl.handle.net/10204/756
ER -
|
en_ZA |