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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/756

Title: Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils
Authors: Lakay, FM
Botha, A
Prior, BA
Keywords: Soil DNA isolation
Liquid nitrogen
Humic acid
Soil
Issue Date: Jan-2007
Publisher: Blackwell Publishing
Citation: Lakay FM, Botha A, Prior BA. 2007. Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils. Journal of Applied Microbiology, 102(1), pp 265-273
Abstract: Three different soil DNA isolation and four purification strategies were compared on different soil samples with variable rates of success. Bead beating extraction gave significantly higher DNA yields than microwave-based and liquid nitrogen grinding DNA extraction methods. The inclusion of soil washing prior to cell lysis decreased the amount of purification steps required. Although these soil types differed, polyvinylpolypyrrolidone (PVPP)-sepharose 2B column elution was sufficient for all three samples, yielding DNA pure enough for successful application in molecular studies. One soil sample retained 80% of the initial DNA after successful purification.
Description: http://www.ncbi.nlm.nih.gov/entrez/query.
URI: http://hdl.handle.net/10204/756
ISSN: 1364-5072
Appears in Collections:General science, engineering & technology
Bioprospecting

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