dc.contributor.author |
Alexandre, Kabamba B
|
|
dc.contributor.author |
Moore, PL
|
|
dc.contributor.author |
Nonyane, M
|
|
dc.contributor.author |
Gray, ES
|
|
dc.contributor.author |
Ranchobe, N
|
|
dc.contributor.author |
Chakauya, E
|
|
dc.contributor.author |
McMahon, JB
|
|
dc.contributor.author |
O'Keefe, BR
|
|
dc.contributor.author |
Chikwamba, Rachel K
|
|
dc.contributor.author |
Morris, L
|
|
dc.date.accessioned |
2013-10-23T11:47:47Z |
|
dc.date.available |
2013-10-23T11:47:47Z |
|
dc.date.issued |
2013-11 |
|
dc.identifier.citation |
Alexandre, K.B, Moore, P.L, Nonyane, M, Gray, E.S, Ranchobe, N, Chakauya, E, McMahon, J.B, O’Keefe, BR and Chikwamba, R. 2013. Morris LMechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN. Virology, vol. 446(1-2), pp 66-76 |
en_US |
dc.identifier.issn |
0042-6822 |
|
dc.identifier.uri |
http://ac.els-cdn.com/S004268221300439X/1-s2.0-S004268221300439X-main.pdf?_tid=52402ad8-34b5-11e3-9e8c-00000aacb35f&acdnat=1381744092_43d11c2ad966a475e41abad428080d4d
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/6981
|
|
dc.description |
Copyright: 2013 Elsevier. This is an ABSTRACT ONLY. The definitive version is published in Virology, vol. 446(1-2), pp 66-76 |
en_US |
dc.description.abstract |
We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured under escalating concentrations of these lectins became increasingly resistant tolerating 2 to 12 times their 50% inhibitory concentrations. Sequence analysis of gp120 showed that most had deletions of 1 to 5 mannose-rich glycans. Glycosylation sites at positions 230, 234, 241, 289 located in the C2 region and 339, 392 and 448 in the C3-C4 region were affected. Furthermore, deletions and insertions of up to 5 amino acids in the V4 region were observed in 3 of the 4 isolates. These data suggest that loss of glycosylation sites on gp120 as well as rearrangement of glycans in V4 are mechanisms involved in HIV-1 subtype C escape from GRFT, CV-N and SVN. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.relation.ispartofseries |
Workflow;11635 |
|
dc.subject |
Griffithsin |
en_US |
dc.subject |
Cyanovirin-N |
en_US |
dc.subject |
Scytovirin |
en_US |
dc.subject |
HIV subtype C |
en_US |
dc.subject |
Entry inhibitors |
en_US |
dc.subject |
Glycans |
en_US |
dc.subject |
Single genome amplification |
en_US |
dc.subject |
Microbicides |
en_US |
dc.subject |
lectins |
en_US |
dc.title |
Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Alexandre, K. B., Moore, P., Nonyane, M., Gray, E., Ranchobe, N., Chakauya, E., ... Morris, L. (2013). Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN. http://hdl.handle.net/10204/6981 |
en_ZA |
dc.identifier.chicagocitation |
Alexandre, Kabamba B, PL Moore, M Nonyane, ES Gray, N Ranchobe, E Chakauya, JB McMahon, BR O'Keefe, Rachel K Chikwamba, and L Morris "Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN." (2013) http://hdl.handle.net/10204/6981 |
en_ZA |
dc.identifier.vancouvercitation |
Alexandre KB, Moore P, Nonyane M, Gray E, Ranchobe N, Chakauya E, et al. Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN. 2013; http://hdl.handle.net/10204/6981. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Alexandre, Kabamba B
AU - Moore, PL
AU - Nonyane, M
AU - Gray, ES
AU - Ranchobe, N
AU - Chakauya, E
AU - McMahon, JB
AU - O'Keefe, BR
AU - Chikwamba, Rachel K
AU - Morris, L
AB - We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured under escalating concentrations of these lectins became increasingly resistant tolerating 2 to 12 times their 50% inhibitory concentrations. Sequence analysis of gp120 showed that most had deletions of 1 to 5 mannose-rich glycans. Glycosylation sites at positions 230, 234, 241, 289 located in the C2 region and 339, 392 and 448 in the C3-C4 region were affected. Furthermore, deletions and insertions of up to 5 amino acids in the V4 region were observed in 3 of the 4 isolates. These data suggest that loss of glycosylation sites on gp120 as well as rearrangement of glycans in V4 are mechanisms involved in HIV-1 subtype C escape from GRFT, CV-N and SVN.
DA - 2013-11
DB - ResearchSpace
DP - CSIR
KW - Griffithsin
KW - Cyanovirin-N
KW - Scytovirin
KW - HIV subtype C
KW - Entry inhibitors
KW - Glycans
KW - Single genome amplification
KW - Microbicides
KW - lectins
LK - https://researchspace.csir.co.za
PY - 2013
SM - 0042-6822
T1 - Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN
TI - Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN
UR - http://hdl.handle.net/10204/6981
ER -
|
en_ZA |