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dc.contributor.author Thwala, Melusi
dc.contributor.author Musee, N
dc.contributor.author Wepener, V
dc.date.accessioned 2013-01-28T07:58:01Z
dc.date.available 2013-01-28T07:58:01Z
dc.date.issued 2012-09
dc.identifier.citation Thwala, M., Musee, N., and Wepener, V. 2012. Zinc oxide and silver nanoparticles influence the antioxidative status in a higher aquatic plant, Spirodela punctata. 7th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials, Banff, Alberta, Canada, 10-12 September 2012 en_US
dc.identifier.uri http://hdl.handle.net/10204/6437
dc.description 7th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials, Banff, Alberta, Canada, 10-12 September 2012 en_US
dc.description.abstract The authors present evidence of free radical activity and resultant anti-oxidative defence in Spirodela plants after exposure to 0.01-1000 mg/L of ZnO and Ag nanoparticles (NPs) over 96-h and 14-d. The quantification of reactive nitrogen and reactive oxygen species (RNS/ROS) revealed elevated (a=0.05) free radical activity only in samples exposed to 0.01 and 0.1 mg/L of ZnO and Ag, respectively, relative to the control over 96-h. The 14-d exposures showed most significant increase of RNS/ROS, and were nanoparticle concentration dependent whilst 100% mortality occurred at the highest Ag NPs exposure. No significant H202 increase over 96-h was observed, however, increases of H202 production under certain nanoparticle concentrations were recorded during the 14-d exposure. The biochemical anti-oxidative status of the plant specimens were investigated using quantitative analysis of total antioxidant capacity, peroxidase and activity of catalase and superoxide dismutase. The anti-oxidative defence mechanisms were non-uniform relative to exposure period and NPs concentration variations; however, evidence of NPs influence was observed. The characterization of NPs using TEM, DLS and XRD, and also exposure water chemistry analysis yielded data that was useful in accounting for the resultant biochemical response. The study highlights the influence of NPs physicochemical properties and environmental factors on nanoecotoxicity. en_US
dc.language.iso en en_US
dc.relation.ispartofseries Workflow;10155
dc.subject Spirodela plants en_US
dc.subject Nanoparticles en_US
dc.subject Nanomaterials en_US
dc.subject Nanomaterials environmental effects en_US
dc.subject Nanoecotoxicity en_US
dc.subject Spirodela en_US
dc.title Zinc oxide and silver nanoparticles influence the antioxidative status in a higher aquatic plant, Spirodela punctata en_US
dc.type Presentation en_US


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