DSpace
 

Researchspace >
General science, engineering & technology >
General science, engineering & technology >
General science, engineering & technology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/6257

Title: Performance evaluation of spot detection algorithms in fluorescence microscopy images
Authors: Mabaso, M
Twala, B
Withey, D
Utete, S
Keywords: Fluorescence microscopy
mRNA
messenger Ribonucleic Acid
Isotropic Undecimated Wavelet Transform
IUWT
Feature Particle Detection
FPD
Algorithms
Particle detection
Noise
Issue Date: Oct-2012
Citation: Mabaso, M, Twala, B, Withey, D and Utete, S. Performance evaluation of spot detection algorithms in fluorescence microscopy images. 4th CSIR Biennial Conference: Real problems relevant solutions, CSIR, Pretoria, 9-10 October 2012
Abstract: Detection of messenger Ribonucleic Acid (mRNA) spots in fluorescence microscopy images is of great importance for biologists seeking better understanding of cell functionality. Fluorescence microscopy and specific staining methods make biological molecules appear as bright spots in image data. Manual analysis of such data is both time-consuming and laborious, and can lead to errors. In this study, we compare two computer-based detection methods used in fluorescence microscopy images for the detection of spots. The algorithms compared are the Isotropic Undecimated Wavelet Transform (IUWT) and the Feature Particle Detection (FPD). The performance of these algorithms is validated on synthetic images. Our study finds a major difference in the performance of the two algorithms. IUWT performs better than FPD.
Description: 4th CSIR Biennial Conference: Real problems relevant solutions, CSIR, Pretoria, 9-10 October 2012
URI: http://hdl.handle.net/10204/6257
Appears in Collections:CSIR Conference 2012
Structural biology
General science, engineering & technology

Files in This Item:

File Description SizeFormat
Mabaso_2012.pdf700.61 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback