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The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases

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dc.contributor.author Kenyon, CP
dc.contributor.author Steyn, A
dc.contributor.author Roth, Robyn L
dc.contributor.author Steenkamp, PA
dc.contributor.author Nkosi, TC
dc.contributor.author Oldfield, LC
dc.date.accessioned 2012-07-05T11:03:25Z
dc.date.available 2012-07-05T11:03:25Z
dc.date.issued 2011-07
dc.identifier.citation Kenyon, CP, Steyn, A, Roth, RL, Steenkamp, PA, Nkosi, TC and Oldfield, LC. 2011. The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases. BMC Biochemistry, vol. 12(36), DOI:10.1186/1471-2091-12-36 en_US
dc.identifier.issn 1471-2091
dc.identifier.uri http://www.biomedcentral.com/content/pdf/1471-2091-12-36.pdf
dc.identifier.uri http://www.biomedcentral.com/content/pdf/1471-2091-12-36.pdf
dc.identifier.uri http://hdl.handle.net/10204/5985
dc.description Copyright: 2011 Kenyon et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.description.abstract Kinase and synthetase enzymes utilise C8D-ATP in preference to non-deuterated ATP. The KIE obtained at low ATP concentrations is clearly a primary KIE demonstrating strong evidence that the bond to the isotopically substituted hydrogen is being broken. The effect of the ATP concentration profile on the KIE was used to develop a model whereby the C8H of ATP plays a role in the overall regulation of phosphoryl transfer. This role of the C8H of ATP in the regulation appears to have been conserved in all kinase and synthetase enzymes as one of the mechanisms associated with binding of ATP. The induction of the C8H to be labile by active site residues coordinated to the ATP purine ring may play a significant role in explaining the broad range of Km associated with kinase enzymes. en_US
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.relation.ispartofseries Workflow;4237
dc.subject Phosphoryl transfer en_US
dc.subject Kinases en_US
dc.subject Synthetases en_US
dc.subject C8 proton en_US
dc.subject Amino acid en_US
dc.subject Enzymes en_US
dc.subject C8D-ATP en_US
dc.title The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases en_US
dc.type Article en_US
dc.identifier.apacitation Kenyon, C., Steyn, A., Roth, R. L., Steenkamp, P., Nkosi, T., & Oldfield, L. (2011). The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases. http://hdl.handle.net/10204/5985 en_ZA
dc.identifier.chicagocitation Kenyon, CP, A Steyn, Robyn L Roth, PA Steenkamp, TC Nkosi, and LC Oldfield "The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases." (2011) http://hdl.handle.net/10204/5985 en_ZA
dc.identifier.vancouvercitation Kenyon C, Steyn A, Roth RL, Steenkamp P, Nkosi T, Oldfield L. The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases. 2011; http://hdl.handle.net/10204/5985. en_ZA
dc.identifier.ris TY - Article AU - Kenyon, CP AU - Steyn, A AU - Roth, Robyn L AU - Steenkamp, PA AU - Nkosi, TC AU - Oldfield, LC AB - Kinase and synthetase enzymes utilise C8D-ATP in preference to non-deuterated ATP. The KIE obtained at low ATP concentrations is clearly a primary KIE demonstrating strong evidence that the bond to the isotopically substituted hydrogen is being broken. The effect of the ATP concentration profile on the KIE was used to develop a model whereby the C8H of ATP plays a role in the overall regulation of phosphoryl transfer. This role of the C8H of ATP in the regulation appears to have been conserved in all kinase and synthetase enzymes as one of the mechanisms associated with binding of ATP. The induction of the C8H to be labile by active site residues coordinated to the ATP purine ring may play a significant role in explaining the broad range of Km associated with kinase enzymes. DA - 2011-07 DB - ResearchSpace DP - CSIR KW - Phosphoryl transfer KW - Kinases KW - Synthetases KW - C8 proton KW - Amino acid KW - Enzymes KW - C8D-ATP LK - https://researchspace.csir.co.za PY - 2011 SM - 1471-2091 T1 - The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases TI - The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases UR - http://hdl.handle.net/10204/5985 ER - en_ZA


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