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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/5848

Title: Theoretical study of bone sialoprotein in bone biomineralization
Authors: Yang, Y
Mkhonto, D
Cui, Q
Sahai, N
Keywords: Bone sialoprotein
Biomineralization
Nucleation
Hydroxyapatite
Computational simulation
Issue Date: May-2011
Publisher: Karger
Citation: Yang, Y, Mkhonto, D, Cui, Q and Sahai, N. 2011. Theoretical study of bone sialoprotein in bone biomineralization. Cells Tissues Organs, vol. 194(2-4), pp 182-187
Series/Report no.: Workflow;8671
Abstract: Bone sialoprotein (BSP) is an acidic, non-collagenous protein specific to bone proposed previously to promote hydroxyapatite (HAP) nucleation and modulate HAP nanocrystal growth. Specifically, two phosphorylated acidic amino acid sequences in BSP, highly conserved across several vertebrates, are the proposed active sites. We selected one of these sites, i.e. (Sp) 2 E 8 , where Sp represents a phosphoserine as a model peptide to study the role of BSP. We used molecular dynamics simulations to determine whether an -helix or a random coil peptide conformation promotes templated HAP nucleation. A bioinformatics method helps infer preferential crystal growth directions by predicting the likely peptide conformations adsorbed on the (001), (100), and (110) crystal faces of HAP. Results suggest that, independent of conformation, no stable nucleating template is formed and, thus, the ion distributions in the vicinity of the peptide that eventually lead to a stable nucleus start out with disordered arrangements of ions. When adsorbed on all three faces, the Sp residues bind strongly regardless of the peptide conformation, and the Glu residues show different propensities to form helical conformations. The lack of geometrical templating between the peptide residues and all HAP surface sites indicates that adsorption and subsequent crystal growth modulation may be structurally nonspecific.
Description: Copyright: 2011 Karger. This is an ABSTRACT ONLY.
URI: http://content.karger.com/ProdukteDB/produkte.asp?Doi=324648
http://hdl.handle.net/10204/5848
ISSN: 1422-6405
Appears in Collections:Accessibility research
Advanced mathematical modelling and simulation
General science, engineering & technology
Bioprospecting
General research interest

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