DSpace
 

Researchspace >
General science, engineering & technology >
General science, engineering & technology >
General science, engineering & technology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/5672

Title: The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells
Authors: Alexandre, KB
Gray, ES
Mufhandu, H
McMahon, JB
Chakauya, E
O'Keefe, BR
Chikwamba, R
Morris, L
Keywords: Griffithsin
Cyanovirin-N
Scytovirin
Mannose-rich glycans
HIV-1 gp120
DC-SIGN receptor
Issue Date: Feb-2012
Publisher: Elsevier
Citation: Alexandre, KB, Gray, ES, Mufhandu, H, McMahon, JB, Chakauya, E, O'Keefe, BR, Chikwamba, R and Morris, L. The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells. Virology, vol. 423(2), pp 175-186
Series/Report no.: Workflow;8494
Abstract: It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) inhibit HIV-1 infection by binding to mannose-rich glycans on gp120. We measured the ability of these lectins to inhibit both the HIV-1 binding to DC-SIGN and the DC-SIGN-mediated HIV-1 infection of CD4(+) cells. While GRFT, CV-N and SVN were moderately inhibitory to DC-SIGN binding, they potently inhibited DC-SIGN-transfer of HIV-1. The introduction of the 234 glycosylation site abolished HIV-1 sensitivity to lectin inhibition of binding to DC-SIGN and virus transfer to susceptible cells. However, the addition of the 295 glycosylation site increased the inhibition of transfer. Our data suggest that GRFT, CV-N and SVN can block two important stages of the sexual transmission of HIV-1, DC-SIGN binding and transfer, supporting their further development as microbicides.
Description: Copyright: 2011 Elsevier. This is an ABSTRACT ONLY.
URI: http://www.sciencedirect.com/science/article/pii/S0042682211005587
http://ac.els-cdn.com/S0042682211005587/1-s2.0-S0042682211005587-main.pdf?_tid=09606c980c7e0bdd929b9dbf9993890a&acdnat=1332490363_5d94f026a31bbf3915b8bad31a4bb6aa
http://hdl.handle.net/10204/5672
ISSN: 0042-6822
Appears in Collections:Human factors
General science, engineering & technology

Files in This Item:

File Description SizeFormat
Chikwamba_2012_ABSTRACT ONLY.pdf126.22 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback