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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/5410

Title: Intracellular dielectric tagging for improved optical manipulation of mammalian cells
Authors: Mthunzi, P
Lee, WM
Riches, AC
Brown, CTA
Gunn-Moore, FJ
Dholakia, K
Keywords: Intracellular dielectric tagging
Optical cell sorting
Axial forces
Scattering forces
Quantum electronics
Mammalian cells
Issue Date: May-2010
Publisher: IEEE
Citation: Mthunzi, P, Lee, WM, Riches, AC et al. 2010. Intracellular dielectric tagging for improved optical manipulation of mammalian cells. IEEE Journal of Selected Topics in Quantum Electronics, Vol 16(3), pp 608-618
Series/Report no.: Workflow request;6085
Abstract: Optical micromanipulation of transparent microparticles such as cellularmaterials relies upon the application of optical forces that are crucially dependent on the refractive index contrast between the particle and the surrounding medium. We briefly review the application of optical forces for cellmanipulation and sorting, highlighting some of the key experiments over the last twenty years.We then introduce a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally taking up microspheres from their environment. We explore how these intracellular dielectric tags can influence the scattering and gradient forces upon these cells from an externally applied optical field. We show that intracellular polymer microspheres can serve as highly directional optical scatterers and that scattering forces can enable sorting through axial guiding onto laminin-coated glass coverslips upon which the selected cells adhere. Such internal dielectric tagging presents a simple, inexpensive, sterile technique to enhance optical manipulation procedures for cellularmaterial and may enable new sorting techniques within microfluidic systems.
Description: Copyright: 2010 IEEE. This is an ABSTRACT ONLY
URI: http://ieeexplore.ieee.org/Xplore/login.jsp?reload=true&url=http%3A%2F%2Fieeexplore.ieee.org%2Fiel5%2F2944%2F5477232%2F05361341.pdf%3Farnumber%3D5361341&authDecision=-203
http://hdl.handle.net/10204/5410
ISSN: 1077-260X
Appears in Collections:Nanotechnology
Laser physics and technology
General science, engineering & technology

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