dc.contributor.author |
Van den Berg, N
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dc.contributor.author |
Mbobela, P
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dc.contributor.author |
Pillay, P
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dc.contributor.author |
London, G
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dc.contributor.author |
Morris, L
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dc.contributor.author |
Maharaj, V
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dc.contributor.author |
Khati, M
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dc.date.accessioned |
2011-07-08T10:50:44Z |
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dc.date.available |
2011-07-08T10:50:44Z |
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dc.date.issued |
2011-02 |
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dc.identifier.citation |
Van den Berg, N, Mbobela, P, Pillay, P et al. 2011. HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide. EMBO Global exchange lecture course, Wallenberg Research Centre, Stellenbosch, February 2011 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/10204/5091
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dc.description |
EMBO Global exchange lecture course, Wallenberg Research Centre, Stellenbosch, February 2011 |
en_US |
dc.description.abstract |
A natural compound isolated from extracts of an indigenous plant in the Eastern Cape, South Africa, indicates neutralisation activity against HIV-1 pseudoviruses. This natural compound can potentially be used in a microbicide as an alternative means to prevent the spread of HIV and AIDS. The compound’s neutralisation activity (IC50 0.05-7.2µg/mL) is comparable with that of the entry inhibitor T-20 (IC50 0.05-2.94µg/mL) and the reverse transcriptase inhibitor Tenofovir (IC50 0.09-0.83µg/mL) when screened against the NIH reference panel for HIV-1 subtype C envelope molecular clones. T20 is a more relevant drug for comparative purposes as it is an entry inhibitor and indications on our compound of interest point to it also acting in a similar manner. The mode of action of the compound is, however, yet to be determined. The aim is to develop a microbicide based on an indigenous plant for people infected with HIV-1. The compound will also be screened against other HIV-1 subtypes to test the neutralisation breadth. The authors have shown that the extracts of the plant and the compound are not cytotoxic towards TZM-bl cells when tested in a MTT assay at concentrations up 100 µg/mL. In addition these did not show any neutralisation activity against VSV-G which has a similar glycoprotein to HIV-1 indicating specificity to HIV-1. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
CSIR |
en_US |
dc.relation.ispartofseries |
Workflow; 5451 |
|
dc.subject |
Natural compounds |
en_US |
dc.subject |
HIV-1 |
en_US |
dc.subject |
HIV-1 pseudovirus neutralisation |
en_US |
dc.subject |
Neutralisation activity |
en_US |
dc.subject |
Indigenous plants |
en_US |
dc.title |
HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide |
en_US |
dc.type |
Conference Presentation |
en_US |
dc.identifier.apacitation |
Van den Berg, N., Mbobela, P., Pillay, P., London, G., Morris, L., Maharaj, V., & Khati, M. (2011). HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide. CSIR. http://hdl.handle.net/10204/5091 |
en_ZA |
dc.identifier.chicagocitation |
Van den Berg, N, P Mbobela, P Pillay, G London, L Morris, V Maharaj, and M Khati. "HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide." (2011): http://hdl.handle.net/10204/5091 |
en_ZA |
dc.identifier.vancouvercitation |
Van den Berg N, Mbobela P, Pillay P, London G, Morris L, Maharaj V, et al, HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide; CSIR; 2011. http://hdl.handle.net/10204/5091 . |
en_ZA |
dc.identifier.ris |
TY - Conference Presentation
AU - Van den Berg, N
AU - Mbobela, P
AU - Pillay, P
AU - London, G
AU - Morris, L
AU - Maharaj, V
AU - Khati, M
AB - A natural compound isolated from extracts of an indigenous plant in the Eastern Cape, South Africa, indicates neutralisation activity against HIV-1 pseudoviruses. This natural compound can potentially be used in a microbicide as an alternative means to prevent the spread of HIV and AIDS. The compound’s neutralisation activity (IC50 0.05-7.2µg/mL) is comparable with that of the entry inhibitor T-20 (IC50 0.05-2.94µg/mL) and the reverse transcriptase inhibitor Tenofovir (IC50 0.09-0.83µg/mL) when screened against the NIH reference panel for HIV-1 subtype C envelope molecular clones. T20 is a more relevant drug for comparative purposes as it is an entry inhibitor and indications on our compound of interest point to it also acting in a similar manner. The mode of action of the compound is, however, yet to be determined. The aim is to develop a microbicide based on an indigenous plant for people infected with HIV-1. The compound will also be screened against other HIV-1 subtypes to test the neutralisation breadth. The authors have shown that the extracts of the plant and the compound are not cytotoxic towards TZM-bl cells when tested in a MTT assay at concentrations up 100 µg/mL. In addition these did not show any neutralisation activity against VSV-G which has a similar glycoprotein to HIV-1 indicating specificity to HIV-1.
DA - 2011-02
DB - ResearchSpace
DP - CSIR
KW - Natural compounds
KW - HIV-1
KW - HIV-1 pseudovirus neutralisation
KW - Neutralisation activity
KW - Indigenous plants
LK - https://researchspace.csir.co.za
PY - 2011
T1 - HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide
TI - HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide
UR - http://hdl.handle.net/10204/5091
ER -
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en_ZA |