dc.contributor.author |
Ashok, AC
|
|
dc.contributor.author |
Marchington, RF
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|
dc.contributor.author |
Mthunzi-Kufa, Patience
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|
dc.contributor.author |
Krauss, TF
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|
dc.contributor.author |
Dholakia, K
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|
dc.date.accessioned |
2011-04-11T08:33:19Z |
|
dc.date.available |
2011-04-11T08:33:19Z |
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dc.date.issued |
2010-03 |
|
dc.identifier.citation |
Ashok, AC, Marchington, RF, Mthunzi, P et al. 2010. Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation. Optics Express, Vol. 18(6), pp 6396-6407 |
en_US |
dc.identifier.issn |
1094-4087 |
|
dc.identifier.uri |
http://www.opticsinfobase.org/view_article.cfm?gotourl=http%3A%2F%2Fwww%2Eopticsinfobase%2Eorg%2FDirectPDFAccess%2F3CD35F9C%2DCA1F%2DEF97%2DA8F24F0164B70D22%5F196601%2Epdf%3Fda%3D1%26id%3D196601%26seq%3D0%26mobile%3Dno&org=
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dc.identifier.uri |
http://hdl.handle.net/10204/4952
|
|
dc.description |
Copyright: 2010 Optical Society of America. |
en_US |
dc.description.abstract |
The authors describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Optical Society of America |
en_US |
dc.relation.ispartofseries |
Workflow;6083 |
|
dc.subject |
Optical chromatography |
en_US |
dc.subject |
On chip fluorescence excitation |
en_US |
dc.subject |
Photonic crystal fiber. |
en_US |
dc.subject |
Optical confinement |
en_US |
dc.subject |
Optical manipulation |
en_US |
dc.subject |
Optical tweezers |
en_US |
dc.subject |
Optics |
en_US |
dc.title |
Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Ashok, A., Marchington, R., Mthunzi, P., Krauss, T., & Dholakia, K. (2010). Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation. http://hdl.handle.net/10204/4952 |
en_ZA |
dc.identifier.chicagocitation |
Ashok, AC, RF Marchington, P Mthunzi, TF Krauss, and K Dholakia "Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation." (2010) http://hdl.handle.net/10204/4952 |
en_ZA |
dc.identifier.vancouvercitation |
Ashok A, Marchington R, Mthunzi P, Krauss T, Dholakia K. Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation. 2010; http://hdl.handle.net/10204/4952. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Ashok, AC
AU - Marchington, RF
AU - Mthunzi, P
AU - Krauss, TF
AU - Dholakia, K
AB - The authors describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging.
DA - 2010-03
DB - ResearchSpace
DP - CSIR
KW - Optical chromatography
KW - On chip fluorescence excitation
KW - Photonic crystal fiber.
KW - Optical confinement
KW - Optical manipulation
KW - Optical tweezers
KW - Optics
LK - https://researchspace.csir.co.za
PY - 2010
SM - 1094-4087
T1 - Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation
TI - Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation
UR - http://hdl.handle.net/10204/4952
ER - |
en_ZA |