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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/4952

Title: Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation
Authors: Ashok, AC
Marchington, RF
Mthunzi, P
Krauss, TF
Dholakia, K
Keywords: Optical chromatography
On chip fluorescence excitation
Photonic crystal fiber.
Optical confinement
Optical manipulation
Optical tweezers
Optics
Issue Date: Mar-2010
Publisher: Optical Society of America
Citation: Ashok, AC, Marchington, RF, Mthunzi, P et al. 2010. Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation. Optics Express, Vol. 18(6), pp 6396-6407
Series/Report no.: Workflow;6083
Abstract: The authors describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging.
Description: Copyright: 2010 Optical Society of America.
URI: http://www.opticsinfobase.org/view_article.cfm?gotourl=http%3A%2F%2Fwww%2Eopticsinfobase%2Eorg%2FDirectPDFAccess%2F3CD35F9C%2DCA1F%2DEF97%2DA8F24F0164B70D22%5F196601%2Epdf%3Fda%3D1%26id%3D196601%26seq%3D0%26mobile%3Dno&org=
http://hdl.handle.net/10204/4952
ISSN: 1094-4087
Appears in Collections:Nanotechnology
Laser physics and technology
General science, engineering & technology

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