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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/4428

Title: High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases
Authors: Visser, DF
Rashamuse, KJ
Hennessy, F
Gordon, GER
Van Zyl, PJ
Mathiba, K
Bode, ML
Brady, D
Louw, ME
Keywords: Purine nucleoside phosphorylase
Uridine phosphorylase
Biocatalysis
5-methyluridine
Fermentation
Transglycosylation
Issue Date: Jul-2010
Publisher: Informa Healthcare
Citation: Visser, DF, Rashamuse, KJ, Hennessy, F et al. 2010. High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases. Biocatalysis and Biotransformation, Vol. 28(4), pp 245–253
Abstract: A novel combination of Bacillus halodurans purine nucleoside phosphorylase (BhPNP1) and Escherichia coli uridine phosphorylase (EcUP) has been applied to a dual-enzyme, sequential, biocatalytic one-pot synthesis of 5-methyluridine from guanosine and thymine. A 5-methyluridine yield of  79% on guanosine was achieved in a reaction slurry at a 53 mM (1.5% w/w) guanosine concentration. 5-Methyluridine is an intermediate in synthetic routes to thymidine and the antiretroviral drugs zidovudine and stavudine.
Description: Copyright: 2010 Informa Healthcare. This is the author's pre print version. The definitive version is published in Biocatalysis and Biotransformation, Vol. 28(4), pp 245–253
URI: http://informahealthcare.com/doi/abs/10.3109/10242422.2010.493210?journalCode=bab
http://hdl.handle.net/10204/4428
ISSN: 1024-2422
Appears in Collections:Enzyme technologies
General science, engineering & technology

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