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dc.contributor.author Van Brummelen, AC
dc.contributor.author Becker, JVW
dc.contributor.author Mancama, Dalubuhle T
dc.contributor.author Hoppe, H
dc.date.accessioned 2010-03-03T09:50:18Z
dc.date.available 2010-03-03T09:50:18Z
dc.date.issued 2010-01
dc.identifier.citation Van Brummelen AC, Becker JVW et al. 2010. Establishing malaria parasite transfection technology in South Africa. 22nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010, pp 2 en
dc.identifier.uri http://hdl.handle.net/10204/3971
dc.description 22nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010 en
dc.description.abstract In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), previously reported to have bidirectional promoter activity, was utilized to drive expression through two genes (i.e. the antibiotic-resistance gene, human dhfr and the reporter gene) in a head-to-head orientation. In addition, protocols were adjusted by including DNA packaging reagents to improve uptake into the parasite and by using shaking instead of stationary parasite cultures to improve the parasite proliferation and selection rate. Successfully transfected parasites were selected by the antifolate WR99210. en
dc.language.iso en en
dc.subject Plasmodium falciparum en
dc.subject Anti-malarial drug en
dc.subject Anti-malarial drug resistance en
dc.subject Transfection en
dc.subject Malaria en
dc.subject Parasite transfection technology en
dc.subject Biochemistry en
dc.subject Molecular biology en
dc.title Establishing malaria parasite transfection technology in South Africa. en
dc.type Poster en


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