dc.contributor.author |
Van Brummelen, AC
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dc.contributor.author |
Becker, JVW
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dc.contributor.author |
Mancama, Dalubuhle T
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dc.contributor.author |
Hoppe, H
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dc.date.accessioned |
2010-03-03T09:50:18Z |
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dc.date.available |
2010-03-03T09:50:18Z |
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dc.date.issued |
2010-01 |
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dc.identifier.citation |
Van Brummelen AC, Becker JVW et al. 2010. Establishing malaria parasite transfection technology in South Africa. 22nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010, pp 2 |
en |
dc.identifier.uri |
http://hdl.handle.net/10204/3971
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|
dc.description |
22nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010 |
en |
dc.description.abstract |
In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), previously reported to have bidirectional promoter activity, was utilized to drive expression through two genes (i.e. the antibiotic-resistance gene, human dhfr and the reporter gene) in a head-to-head orientation. In addition, protocols were adjusted by including DNA packaging reagents to improve uptake into the parasite and by using shaking instead of stationary parasite cultures to improve the parasite proliferation and selection rate. Successfully transfected parasites were selected by the antifolate WR99210. |
en |
dc.language.iso |
en |
en |
dc.subject |
Plasmodium falciparum |
en |
dc.subject |
Anti-malarial drug |
en |
dc.subject |
Anti-malarial drug resistance |
en |
dc.subject |
Transfection |
en |
dc.subject |
Malaria |
en |
dc.subject |
Parasite transfection technology |
en |
dc.subject |
Biochemistry |
en |
dc.subject |
Molecular biology |
en |
dc.title |
Establishing malaria parasite transfection technology in South Africa. |
en |
dc.type |
Conference Presentation |
en |
dc.identifier.apacitation |
Van Brummelen, A., Becker, J., Mancama, D. T., & Hoppe, H. (2010). Establishing malaria parasite transfection technology in South Africa. http://hdl.handle.net/10204/3971 |
en_ZA |
dc.identifier.chicagocitation |
Van Brummelen, AC, JVW Becker, Dalubuhle T Mancama, and H Hoppe. "Establishing malaria parasite transfection technology in South Africa." (2010): http://hdl.handle.net/10204/3971 |
en_ZA |
dc.identifier.vancouvercitation |
Van Brummelen A, Becker J, Mancama DT, Hoppe H, Establishing malaria parasite transfection technology in South Africa; 2010. http://hdl.handle.net/10204/3971 . |
en_ZA |
dc.identifier.ris |
TY - Conference Presentation
AU - Van Brummelen, AC
AU - Becker, JVW
AU - Mancama, Dalubuhle T
AU - Hoppe, H
AB - In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), previously reported to have bidirectional promoter activity, was utilized to drive expression through two genes (i.e. the antibiotic-resistance gene, human dhfr and the reporter gene) in a head-to-head orientation. In addition, protocols were adjusted by including DNA packaging reagents to improve uptake into the parasite and by using shaking instead of stationary parasite cultures to improve the parasite proliferation and selection rate. Successfully transfected parasites were selected by the antifolate WR99210.
DA - 2010-01
DB - ResearchSpace
DP - CSIR
KW - Plasmodium falciparum
KW - Anti-malarial drug
KW - Anti-malarial drug resistance
KW - Transfection
KW - Malaria
KW - Parasite transfection technology
KW - Biochemistry
KW - Molecular biology
LK - https://researchspace.csir.co.za
PY - 2010
T1 - Establishing malaria parasite transfection technology in South Africa
TI - Establishing malaria parasite transfection technology in South Africa
UR - http://hdl.handle.net/10204/3971
ER -
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en_ZA |