dc.contributor.author |
Mufhandu, Hazel T
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dc.contributor.author |
Madiga, M
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dc.contributor.author |
Gray, E
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dc.contributor.author |
Rotherham, L
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dc.contributor.author |
Khoza, T
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dc.contributor.author |
Morris, L
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dc.contributor.author |
Khati, M
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dc.date.accessioned |
2010-03-03T09:24:24Z |
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dc.date.available |
2010-03-03T09:24:24Z |
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dc.date.issued |
2009-07 |
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dc.identifier.citation |
Mufhandu H, Madiga, M et al., 2009. Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1. 5th International AIDS Society Conference on HIV Pathogenesis Treatment and Prevention. Cape Town, South Africa, 19-22 July 2009, pp 1 |
en |
dc.identifier.uri |
http://hdl.handle.net/10204/3970
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dc.description |
5th International AIDS Society Conference on HIV Pathogenesis Treatment and Prevention. Cape Town, South Africa, 19-22 July 2009 |
en |
dc.description.abstract |
This paper present a chemically synthesised derivative of the B40 parental aptamer, called UCLA1 (Cohen et al., 2008), was used for neutralization of endemic subtype C clinical isolates of HIV-1 from adult and paediatric patients and subtype B lab adapted strains. The UCLA1 RNA aptamer was used for neutralization of a panel of HIV-1 subtype C and HIV-1 subtype B Env-pseudotyped viruses. Neutralization of HIV-1 was measured in duplicate as a reduction in luciferase gene expression after a single-round infection of TZM-bl cells with the Env-pseudotyped viruses as previously described (Gray et al., 2006). The binding affinity and dissociation constant of the aptamer were measured against HIV-1 subtype C Du151 gp120 glycoprotein using the BIAcore Surface Plasmon Resonance Technology (SPR) technology. Cytotoxicity of the aptamer was measured overnight in TZM-bl cells using an ATP-based luminescent assay. The therapeutic index (TI) of the aptamer was determined at a CC50 of 500nM for all the viruses tested |
en |
dc.language.iso |
en |
en |
dc.subject |
UCLA1 RNA aptamer |
en |
dc.subject |
UCLA1 |
en |
dc.subject |
HIV/AIDS |
en |
dc.subject |
HIV-1 |
en |
dc.subject |
HIV pathogenesis |
en |
dc.title |
Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1. |
en |
dc.type |
Conference Presentation |
en |
dc.identifier.apacitation |
Mufhandu, H. T., Madiga, M., Gray, E., Rotherham, L., Khoza, T., Morris, L., & Khati, M. (2009). Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1. http://hdl.handle.net/10204/3970 |
en_ZA |
dc.identifier.chicagocitation |
Mufhandu, Hazel T, M Madiga, E Gray, L Rotherham, T Khoza, L Morris, and M Khati. "Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1." (2009): http://hdl.handle.net/10204/3970 |
en_ZA |
dc.identifier.vancouvercitation |
Mufhandu HT, Madiga M, Gray E, Rotherham L, Khoza T, Morris L, et al, Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1; 2009. http://hdl.handle.net/10204/3970 . |
en_ZA |
dc.identifier.ris |
TY - Conference Presentation
AU - Mufhandu, Hazel T
AU - Madiga, M
AU - Gray, E
AU - Rotherham, L
AU - Khoza, T
AU - Morris, L
AU - Khati, M
AB - This paper present a chemically synthesised derivative of the B40 parental aptamer, called UCLA1 (Cohen et al., 2008), was used for neutralization of endemic subtype C clinical isolates of HIV-1 from adult and paediatric patients and subtype B lab adapted strains. The UCLA1 RNA aptamer was used for neutralization of a panel of HIV-1 subtype C and HIV-1 subtype B Env-pseudotyped viruses. Neutralization of HIV-1 was measured in duplicate as a reduction in luciferase gene expression after a single-round infection of TZM-bl cells with the Env-pseudotyped viruses as previously described (Gray et al., 2006). The binding affinity and dissociation constant of the aptamer were measured against HIV-1 subtype C Du151 gp120 glycoprotein using the BIAcore Surface Plasmon Resonance Technology (SPR) technology. Cytotoxicity of the aptamer was measured overnight in TZM-bl cells using an ATP-based luminescent assay. The therapeutic index (TI) of the aptamer was determined at a CC50 of 500nM for all the viruses tested
DA - 2009-07
DB - ResearchSpace
DP - CSIR
KW - UCLA1 RNA aptamer
KW - UCLA1
KW - HIV/AIDS
KW - HIV-1
KW - HIV pathogenesis
LK - https://researchspace.csir.co.za
PY - 2009
T1 - Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1
TI - Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1
UR - http://hdl.handle.net/10204/3970
ER -
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en_ZA |