DSpace
 

Researchspace >
General science, engineering & technology >
General science, engineering & technology >
General science, engineering & technology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/3970

Title: Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1.
Authors: Mufhandu, H
Madiga, M
Gray, E
Rotherham, L
Khoza, T
Morris, L
Khati, M
Keywords: UCLA1 RNA aptamer
UCLA1
HIV/AIDS
HIV-1
HIV pathogenesis
Issue Date: Jul-2009
Citation: Mufhandu H, Madiga, M et al., 2009. Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1. 5th International AIDS Society Conference on HIV Pathogenesis Treatment and Prevention. Cape Town, South Africa, 19-22 July 2009, pp 1
Abstract: This paper present a chemically synthesised derivative of the B40 parental aptamer, called UCLA1 (Cohen et al., 2008), was used for neutralization of endemic subtype C clinical isolates of HIV-1 from adult and paediatric patients and subtype B lab adapted strains. The UCLA1 RNA aptamer was used for neutralization of a panel of HIV-1 subtype C and HIV-1 subtype B Env-pseudotyped viruses. Neutralization of HIV-1 was measured in duplicate as a reduction in luciferase gene expression after a single-round infection of TZM-bl cells with the Env-pseudotyped viruses as previously described (Gray et al., 2006). The binding affinity and dissociation constant of the aptamer were measured against HIV-1 subtype C Du151 gp120 glycoprotein using the BIAcore Surface Plasmon Resonance Technology (SPR) technology. Cytotoxicity of the aptamer was measured overnight in TZM-bl cells using an ATP-based luminescent assay. The therapeutic index (TI) of the aptamer was determined at a CC50 of 500nM for all the viruses tested
Description: 5th International AIDS Society Conference on HIV Pathogenesis Treatment and Prevention. Cape Town, South Africa, 19-22 July 2009
URI: http://hdl.handle.net/10204/3970
Appears in Collections:General science, engineering & technology
Aptamer technology

Files in This Item:

File Description SizeFormat
Mufhandu_2009.pdf722.08 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback