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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/3015

Title: Heterologous expression of plasmodial proteins for structural studies and functional annotation
Authors: Birkholtz, LM
Blatch, G
Coetzer, TL
Hoppe, HC
Human, E
Morris, EJ
Ngcete, Z
Oldfield, LC
Roth, RL
Shonhai, A
Stephens, L
Louw, AI
Keywords: Antimalarial drugs
Parasite proteins
Plasmodial proteins
Issue Date: 2008
Publisher: BioMed Central
Citation: Birkholtz, L-M, Blatch, G, Coetzer, TL et al. 2008. Heterologous expression of plasmodial proteins for structural studies and functional annotation. Malaria Journal, Vol 7(197), pp 1-20
Abstract: Malaria remains the world's most devastating tropical infectious disease with as many as 40% of the world population living in risk areas. The widespread resistance of plasmodium parasites to the cost-effective chloroquine and antifolates has forced the introduction of more costly drug combinations, such as Coartem. In the absence of a vaccine in the foreseeable future, one strategy to address the growing malaria problem is to identify and characterize new and durable anti-malarial drug targets, the majority of which are parasite proteins. Biochemical and structure-activity analysis of these proteins is ultimately essential in the characterization of such targets but requires large amounts of functional protein. Even though heterologous protein production has now become a relatively routine endeavour for most proteins of diverse origins, the functional expression of soluble plasmodial proteins is highly problematic and slows the progress of anti-malarial drug target discovery. Here the status quo of heterologous production of plasmodial proteins is presented, constraints are highlighted and alternative strategies and hosts for functional expression and annotation of plasmodial proteins are reviewed
Description: Copyright: 2008 BioMed Central Ltd
URI: http://hdl.handle.net/10204/3015
ISSN: 1475-2875
Appears in Collections:Microbial expression systems
Structural biology
General science, engineering & technology

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