N-terminal sequencing gave rise to homology to flagellin protein, product of the hag gene. protein, product of the hag gene. Gene was cloned by using degenerate primers and inverse PCR. The gene sequence as well as the up- and down- stream regions was found to be 100% homologous at the nucleotide level to B halodurans C125
Reference:
Du Plessis, A, Theron, J, Berger, E, and Louw, ME. 2006. Cell surface expression system for the display of heterologous gene products using chimeric flagellin fusions of bacillus halodurans isolate. The 4th Recombinant Protein Production Meeting: a comparative view on host physiology Barcelona, Spain. 21–23 September 2006 - Microbial Cell Factories, 5(Suppl 1), pp 84
Du Plessis, A., Theron, J., Berger, E., & Louw, M. (2006). Cell surface expression system for the display of heterologous gene products using chimeric flagellin fusions of bacillus halodurans isolate. http://hdl.handle.net/10204/2897
Du Plessis, A, J Theron, E Berger, and ME Louw. "Cell surface expression system for the display of heterologous gene products using chimeric flagellin fusions of bacillus halodurans isolate." (2006): http://hdl.handle.net/10204/2897
Du Plessis A, Theron J, Berger E, Louw M, Cell surface expression system for the display of heterologous gene products using chimeric flagellin fusions of bacillus halodurans isolate; 2006. http://hdl.handle.net/10204/2897 .
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