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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/2866

Title: Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica
Authors: Kahari, D
Koorsen, G
Stoychev, S
Chipeta, Z
Labuschagne, M
Van Zyl, P
Nthangeni, B
Keywords: Protein
Yarrowia lipolytica yeast
N-linked glycans
Issue Date: Nov-2008
Publisher: CSIR
Citation: Kahari, D, Koorsen, G, Stoychev, S et al. 2008. Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica. Science Real and Relevant: 2nd CSIR Biennial Conference, CSIR International Convention Centre Pretoria, 17 & 18 November 2008, pp 1
Abstract: The CSIR Biosciences researchers are developing a platform for robust techniques for glyco-profiling of therapeutic glycoproteins. The extracellular lipase (Lip2) (Aloulou, A et al;2007) was used as a model in establishing and evaluating glycan profiling techniques. The gene encoding Lip2 was cloned as a C-terminally His-tagged protein, expressed in Yarrowia lipolytica (Madzak, C et al;2004) and the glycan composition of the purified protein was analysed by HPLC and MALDITOF. The HPLC techniques was also used to profile the glycan composition of EPO expressed in Yarrowia lipolytica yeast.
Description: Science Real and Relevant: 2nd CSIR Biennial Conference, CSIR International Convention Centre Pretoria, 17 & 18 November 2008
URI: http://hdl.handle.net/10204/2866
Appears in Collections:CSIR Conference 2008
General science, engineering & technology

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