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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/2180

Title: Use of monoclonal-antibodies for the detection of fecal bacteria in water
Authors: Kfir, R
Du Preez, M
Genthe, B
Keywords: Monoclonal antibodies
Escherichia coli
Klebsiella
Fecal coliform
Issue Date: 1993
Publisher: Pergamon-Elsevier Science Ltd
Citation: Kfir, R, Du Preez, M and Genthe, B. 1993. Use of monoclonal-antibodies for the detection of fecal bacteria in water. Water Science and Technology, vol. 27, 04 March, pp 257-260
Abstract: Monoclonal antibodies (MAbs) against heat-killed Escherichia coli and Klebsiella oxytoca originating from wastewater effluent were raised in BALB/C mice. The fusion was highly successful and three hybridomas cloned were selected to study the affinity and specificity pattern of the MAbs. The MAbs were found to react equally well with heat-killed and live bacteria when tested against their original immunogens. The MAbs showed lower specificity towards environmentally isolated faecal bacteria than a pure bacterial strain grown in the laboratory. A commercially obtained MAb showed the same specificity pattern.
URI: http://hdl.handle.net/10204/2180
http://hdl.handle.net/10204/2180
ISSN: 0273-1223
Appears in Collections:Pollution and waste
Water resources and human health
General science, engineering & technology

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