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Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis

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dc.contributor.author Louw, M en_US
dc.contributor.author Reid, SJ en_US
dc.contributor.author Watson, TG en_US
dc.date.accessioned 2007-03-26T06:56:36Z en_US
dc.date.accessioned 2007-06-07T10:10:00Z
dc.date.available 2007-03-26T06:56:36Z en_US
dc.date.available 2007-06-07T10:10:00Z
dc.date.issued 1993-01 en_US
dc.identifier.citation Louw, M, Reid, SJ, and Watson, TG. 1993. Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis. Applied Microbiology and Biotechnology, vol. 38(4), pp 507-513 en_US
dc.identifier.issn 0175-7598 en_US
dc.identifier.uri http://hdl.handle.net/10204/2023 en_US
dc.identifier.uri http://hdl.handle.net/10204/2023
dc.description.abstract A Bacillus brevis gene coding for an endo-(1, 3-1, 4)-beta-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the beta-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1, 3-1, 4)-beta-glucanases. The optimum temperature and pH for enzyme activity were 65-70-degrees-C and 8-10, respectively. When held at 75-degrees-C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS. en_US
dc.format.extent 792277 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en en_US
dc.publisher Springer Verlag en_US
dc.rights Copyright: 1993 Springer Verlag en_US
dc.subject Bacillus brevis en_US
dc.subject Escherichia coli en_US
dc.subject Beta-glucanase en_US
dc.subject Applied microbiology en_US
dc.title Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis en_US
dc.type Article en_US
dc.identifier.apacitation Louw, M., Reid, S., & Watson, T. (1993). Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis. http://hdl.handle.net/10204/2023 en_ZA
dc.identifier.chicagocitation Louw, M, SJ Reid, and TG Watson "Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis." (1993) http://hdl.handle.net/10204/2023 en_ZA
dc.identifier.vancouvercitation Louw M, Reid S, Watson T. Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis. 1993; http://hdl.handle.net/10204/2023. en_ZA
dc.identifier.ris TY - Article AU - Louw, M AU - Reid, SJ AU - Watson, TG AB - A Bacillus brevis gene coding for an endo-(1, 3-1, 4)-beta-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the beta-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1, 3-1, 4)-beta-glucanases. The optimum temperature and pH for enzyme activity were 65-70-degrees-C and 8-10, respectively. When held at 75-degrees-C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS. DA - 1993-01 DB - ResearchSpace DP - CSIR KW - Bacillus brevis KW - Escherichia coli KW - Beta-glucanase KW - Applied microbiology LK - https://researchspace.csir.co.za PY - 1993 SM - 0175-7598 T1 - Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis TI - Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis UR - http://hdl.handle.net/10204/2023 ER - en_ZA


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