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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/2023

Title: Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis
Authors: Louw, M
Reid, SJ
Watson, TG
Keywords: Bacillus brevis
Escherichia coli
Beta-glucanase
Applied microbiology
Issue Date: Jan-1993
Publisher: Springer Verlag
Citation: Louw, M, Reid, SJ, and Watson, TG. 1993. Characterization, cloning and sequencing of a thermostable endo-(1, 3-1, 4) beta-glucanase-encoding gene from an alkalophilic Bacillus-brevis. Applied Microbiology and Biotechnology, vol. 38(4), pp 507-513
Abstract: A Bacillus brevis gene coding for an endo-(1, 3-1, 4)-beta-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the beta-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1, 3-1, 4)-beta-glucanases. The optimum temperature and pH for enzyme activity were 65-70-degrees-C and 8-10, respectively. When held at 75-degrees-C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.
URI: http://hdl.handle.net/10204/2023
http://hdl.handle.net/10204/2023
ISSN: 0175-7598
Appears in Collections:Microbial expression systems
General science, engineering & technology

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