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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/2022

Title: Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis
Authors: Louw, M
Reid, SJ
James, DM
Watson, TG
Keywords: Bacillus brevis
Sequence comparisons
Amino acid
Applied microbiology
Issue Date: Oct-1994
Publisher: Springer Verlag
Citation: Louw, M, et al. 1994. Cloning and sequencing the degs-degu operon from an alkalophilic Bacillus-brevis. Applied Microbiology and Biotechnology, vol. 42(1), pp 78-84
Abstract: The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec (-) strain as well as in a B. subtilis sacU (HY) strain.
URI: http://hdl.handle.net/10204/2022
ISSN: 0175-7598
Appears in Collections:General science, engineering & technology

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