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http://hdl.handle.net/10204/2022
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| Title: | Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis |
| Authors: | Louw, M Reid, SJ James, DM Watson, TG |
| Keywords: | Bacillus brevis DegS-degU Sequence comparisons Amino acid Applied microbiology |
| Issue Date: | Oct-1994 |
| Publisher: | Springer Verlag |
| Citation: | Louw, M, et al. 1994. Cloning and sequencing the degs-degu operon from an alkalophilic Bacillus-brevis. Applied Microbiology and Biotechnology, vol. 42(1), pp 78-84 |
| Abstract: | The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec (-) strain as well as in a B. subtilis sacU (HY) strain. |
| URI: | http://hdl.handle.net/10204/2022 http://hdl.handle.net/10204/2022 |
| ISSN: | 0175-7598 |
| Appears in Collections: | Bioprospecting General science, engineering & technology
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