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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/1664

Title: Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase
Authors: O'Kennedy, MM
Burger, JT
Botha, FC
Keywords: Immature zygotic embryos
Biolistics
Pennisetum glaucum (L.) R. Br
Pearl millet
Transgenic maize plants
Plant sciences
Issue Date: Apr-2004
Publisher: Springer-Verlag
Citation: O'Kennedy, MM, Burger, JT and Botha, FC. Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase. Plant Cell Reports, vol. 22(9), pp 684-690
Abstract: Fertile transgenic pearl millet plants expressing a phosphomannose isomerase (PMI) transgene under control of the maize ubiquitin constitutive promoter were obtained using the transformation system described here. Proliferating immature zygotic embryos were used as target tissue for bombardment using a particle inflow gun. Different culture and selection strategies were assessed in order to obtain an optimised mannose selection protocol. Stable integration of the manA gene into the genome of pearl millet was confirmed by PCR and Southern blot analysis. Stable integration of the manA transgene into the genome of pearl millet was demonstrated in T-1 and T-2 progeny of two independent transformation events with no more than four to ten copies of the transgene. Similar to results obtained from previous studies with maize and wheat, the manA gene was shown to be a superior selectable marker gene for improving transformation efficiencies when compared to antibiotic or herbicide selectable marker genes.
URI: http://hdl.handle.net/10204/1664
http://hdl.handle.net/10204/1664
ISSN: 0721-7714
Appears in Collections:Plant biotechnology
General science, engineering & technology

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