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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/1516

Title: Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane
Authors: Maritz, J
Krieg, HM
Yeates, CA
Botes, AL
Breytenbach, JC
Keywords: Calcium alginates
Epoxide hydrolases
Cell immobilisation
Rhodosporidium toruloides
(R,S)-1,2-epoxyoctane
Issue Date: Oct-2003
Publisher: Kluwer Academic Publisher
Citation: Maritz, J, et al. 2003. Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane. Biotechnology Letters, vol 25 (20), pp 1775-1781
Abstract: Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilized in calcium alginate beads for the enantio selective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilized biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M was utilized for bead preparation.
URI: http://hdl.handle.net/10204/1516
http://hdl.handle.net/10204/1516
ISSN: 0141-5492
Appears in Collections:Yeast expression systems
General science, engineering & technology

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