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Quantification of viral particles using a photonic crystal biosensor

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dc.contributor.author Lugongolo, Masixole Y
dc.contributor.author Ombinda-Lemboumba, Saturnin
dc.contributor.author Maphanga, Charles P
dc.contributor.author Mcoyi, Phumulani M
dc.contributor.author El-Hussein, A
dc.contributor.author Mthunzi-Kufa, Patience
dc.date.accessioned 2024-06-11T08:22:11Z
dc.date.available 2024-06-11T08:22:11Z
dc.date.issued 2024-03
dc.identifier.citation Lugongolo, M.Y., Ombinda-Lemboumba, S., Maphanga, C.P., Mcoyi, P.M., El-Hussein, A. & Mthunzi-Kufa, P. 2024. Quantification of viral particles using a photonic crystal biosensor. http://hdl.handle.net/10204/13686 . en_ZA
dc.identifier.issn 0277-786X
dc.identifier.uri https://doi.org/10.1117/12.3002395
dc.identifier.uri http://hdl.handle.net/10204/13686
dc.description.abstract The quantification of human immunodeficiency virus at point of care remains a challenge in resource limited settings. The incorporation of nanotechnology and label free optical biosensing has unlocked promising opportunities in the development of diagnostic tools for infectious diseases. Optical biosensors offer a rapid and sensitive optical method for various biological materials such as cells, biomolecules, and viruses by monitoring the dielectric permittivity changes at the interface of a transducer substrate and the analyte. This work focuses on exploring photonic crystal biosensor efficiency and sensitivity for viral load measurement. Photonic crystal biosensors are a unique class of biosensors that allow for label free analysis as they can control and confine light propagation due to the photonic bandgap. Silane treated photonic crystal was functionalized with anti-HIV-gp120 antibody before the addition of various concentrations of HIV pseudovirus. The samples were analyzed on a custom build transmission spectroscopy that used white light as a light source. The results showed a red shift at different virus concentrations, which demonstrates that photonic crystal biosensors are sensitive enough to detect differences in virus concentrations. Therefore, photonic crystals have a potential in the development of photonic crystal-based biosensors for viral load detection. en_US
dc.format Abstract en_US
dc.language.iso en en_US
dc.relation.uri https://spie.org/photonics-west/presentation/Quantification-of-viral-particles-using-a-photonic-crystal-biosensor/12860-11#_=_ en_US
dc.source Proceedings of SPIE 12860: Plasmonics in Biology and Medicine XXI; 1286002, San Francisco, California, United States, January 2024 en_US
dc.subject Photonics crystal en_US
dc.subject Human immunodeficiency virus en_US
dc.subject HIV en_US
dc.subject Viral load en_US
dc.subject Optical biosensor en_US
dc.title Quantification of viral particles using a photonic crystal biosensor en_US
dc.type Conference Presentation en_US
dc.description.pages 7 en_US
dc.description.note © 2024 SPIE. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website: https://doi.org/10.1117/12.3002395 en_US
dc.description.cluster Manufacturing en_US
dc.description.impactarea Biophotonics en_US
dc.identifier.apacitation Lugongolo, M. Y., Ombinda-Lemboumba, S., Maphanga, C. P., Mcoyi, P. M., El-Hussein, A., & Mthunzi-Kufa, P. (2024). Quantification of viral particles using a photonic crystal biosensor. http://hdl.handle.net/10204/13686 en_ZA
dc.identifier.chicagocitation Lugongolo, Masixole Y, Saturnin Ombinda-Lemboumba, Charles P Maphanga, Phumulani M Mcoyi, A El-Hussein, and Patience Mthunzi-Kufa. "Quantification of viral particles using a photonic crystal biosensor." <i>Proceedings of SPIE 12860: Plasmonics in Biology and Medicine XXI; 1286002, San Francisco, California, United States, January 2024</i> (2024): http://hdl.handle.net/10204/13686 en_ZA
dc.identifier.vancouvercitation Lugongolo MY, Ombinda-Lemboumba S, Maphanga CP, Mcoyi PM, El-Hussein A, Mthunzi-Kufa P, Quantification of viral particles using a photonic crystal biosensor; 2024. http://hdl.handle.net/10204/13686 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Lugongolo, Masixole Y AU - Ombinda-Lemboumba, Saturnin AU - Maphanga, Charles P AU - Mcoyi, Phumulani M AU - El-Hussein, A AU - Mthunzi-Kufa, Patience AB - The quantification of human immunodeficiency virus at point of care remains a challenge in resource limited settings. The incorporation of nanotechnology and label free optical biosensing has unlocked promising opportunities in the development of diagnostic tools for infectious diseases. Optical biosensors offer a rapid and sensitive optical method for various biological materials such as cells, biomolecules, and viruses by monitoring the dielectric permittivity changes at the interface of a transducer substrate and the analyte. This work focuses on exploring photonic crystal biosensor efficiency and sensitivity for viral load measurement. Photonic crystal biosensors are a unique class of biosensors that allow for label free analysis as they can control and confine light propagation due to the photonic bandgap. Silane treated photonic crystal was functionalized with anti-HIV-gp120 antibody before the addition of various concentrations of HIV pseudovirus. The samples were analyzed on a custom build transmission spectroscopy that used white light as a light source. The results showed a red shift at different virus concentrations, which demonstrates that photonic crystal biosensors are sensitive enough to detect differences in virus concentrations. Therefore, photonic crystals have a potential in the development of photonic crystal-based biosensors for viral load detection. DA - 2024-03 DB - ResearchSpace DP - CSIR J1 - Proceedings of SPIE 12860: Plasmonics in Biology and Medicine XXI; 1286002, San Francisco, California, United States, January 2024 KW - Photonics crystal KW - Human immunodeficiency virus KW - HIV KW - Viral load KW - Optical biosensor LK - https://researchspace.csir.co.za PY - 2024 SM - 0277-786X T1 - Quantification of viral particles using a photonic crystal biosensor TI - Quantification of viral particles using a photonic crystal biosensor UR - http://hdl.handle.net/10204/13686 ER - en_ZA
dc.identifier.worklist 27857 en_US


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