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Biosensing multidrug-resistant TB genes using SPR

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dc.contributor.author Chauke, Sipho H
dc.contributor.author Ombinda-Lemboumba, Saturnin
dc.contributor.author Dube, FS
dc.contributor.author Mthunzi-Kufa, Patience
dc.date.accessioned 2024-05-28T11:10:30Z
dc.date.available 2024-05-28T11:10:30Z
dc.date.issued 2024-01
dc.identifier.citation Chauke, S.H., Ombinda-Lemboumba, S., Dube, F. & Mthunzi-Kufa, P. 2024. Biosensing multidrug-resistant TB genes using SPR. http://hdl.handle.net/10204/13675 . en_ZA
dc.identifier.isbn 9781510669390
dc.identifier.issn 1605-7422
dc.identifier.uri http://hdl.handle.net/10204/13675
dc.description.abstract Tuberculosis (TB) is one of the most prevalent infectious diseases globally. Although it is curable, several factors, such as the inappropriate use of treatment drugs lead to drug-resistant strains of TB. The burden of infection is disproportionately high in low-income and resource-limited settings. Furthermore, this disparity is exacerbated in patients with already compromised immune systems. Therefore, early detection and treatment of TB play an important role in reducing the spread and progression to drug-resistant disease forms. There are currently a few rapid multi-drug resistant TB diagnostic tests available, however, most are limited due to costs and accessibility. Several genes, such as catalase-peroxidase (katG) and enoyl reductase (inhA) genes, contain mutations that are responsible for resistance to the TB drug, isoniazid. We therefore, aim to use a custom-built surface plasmon resonance (SPR) system to detect katG and inhA genes. Deoxyribonucleic acid (DNA) probes, specific for katG and inhA, were used as biorecognition elements to capture katG and inhA target DNA. The katG and inhA gene-specific DNA probes were immobilized on a gold-coated glass sensor chip before the target DNA was introduced for detection. As a negative control, a mismatched probe, unspecific to both genes was used for confirmation of the absence of the two genes in the experimental setup. The specificity and sensitivity of the capture probes to the target DNA were investigated using the gold-coated glass sensor chip on the SPR setup. The changes in the resonance angle dip indicated the hybridization of the target DNA and the capture probe. The results from this study will contribute to the optimization of an optical-based biosensor detecting drug-resistant mutations. en_US
dc.format Abstract en_US
dc.language.iso en en_US
dc.relation.uri https://spie.org/photonics-west/presentation/Biosensing-multidrug-resistant-TB-genes-using-SPR/12840-39#_=_ en_US
dc.relation.uri https://spie.org/PW24B/conferencedetails/optical-interactions-tissues-cells#_=_ en_US
dc.source Proceedings of SPIE Conference 12840 - Optical Interactions with Tissue and Cells XXXV, San Francisco, California, United States, 27-28 January 2024 en_US
dc.subject Multidrug-resistance en_US
dc.subject Tuberculosis en_US
dc.subject TB en_US
dc.subject Sensor chip en_US
dc.subject Probes en_US
dc.subject Hybridization en_US
dc.subject Surface Plasmon resonance en_US
dc.title Biosensing multidrug-resistant TB genes using SPR en_US
dc.type Conference Presentation en_US
dc.description.pages 8 en_US
dc.description.note © 2024 SPIE. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website: https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12840/3002305/Biosensing-multidrug-resistant-TB-genes-using-SPR/10.1117/12.3002305.full#_=_ en_US
dc.description.cluster Manufacturing en_US
dc.description.impactarea Biophotonics en_US
dc.identifier.apacitation Chauke, S. H., Ombinda-Lemboumba, S., Dube, F., & Mthunzi-Kufa, P. (2024). Biosensing multidrug-resistant TB genes using SPR. http://hdl.handle.net/10204/13675 en_ZA
dc.identifier.chicagocitation Chauke, Sipho H, Saturnin Ombinda-Lemboumba, FS Dube, and Patience Mthunzi-Kufa. "Biosensing multidrug-resistant TB genes using SPR." <i>Proceedings of SPIE Conference 12840 - Optical Interactions with Tissue and Cells XXXV, San Francisco, California, United States, 27-28 January 2024</i> (2024): http://hdl.handle.net/10204/13675 en_ZA
dc.identifier.vancouvercitation Chauke SH, Ombinda-Lemboumba S, Dube F, Mthunzi-Kufa P, Biosensing multidrug-resistant TB genes using SPR; 2024. http://hdl.handle.net/10204/13675 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Chauke, Sipho H AU - Ombinda-Lemboumba, Saturnin AU - Dube, FS AU - Mthunzi-Kufa, Patience AB - Tuberculosis (TB) is one of the most prevalent infectious diseases globally. Although it is curable, several factors, such as the inappropriate use of treatment drugs lead to drug-resistant strains of TB. The burden of infection is disproportionately high in low-income and resource-limited settings. Furthermore, this disparity is exacerbated in patients with already compromised immune systems. Therefore, early detection and treatment of TB play an important role in reducing the spread and progression to drug-resistant disease forms. There are currently a few rapid multi-drug resistant TB diagnostic tests available, however, most are limited due to costs and accessibility. Several genes, such as catalase-peroxidase (katG) and enoyl reductase (inhA) genes, contain mutations that are responsible for resistance to the TB drug, isoniazid. We therefore, aim to use a custom-built surface plasmon resonance (SPR) system to detect katG and inhA genes. Deoxyribonucleic acid (DNA) probes, specific for katG and inhA, were used as biorecognition elements to capture katG and inhA target DNA. The katG and inhA gene-specific DNA probes were immobilized on a gold-coated glass sensor chip before the target DNA was introduced for detection. As a negative control, a mismatched probe, unspecific to both genes was used for confirmation of the absence of the two genes in the experimental setup. The specificity and sensitivity of the capture probes to the target DNA were investigated using the gold-coated glass sensor chip on the SPR setup. The changes in the resonance angle dip indicated the hybridization of the target DNA and the capture probe. The results from this study will contribute to the optimization of an optical-based biosensor detecting drug-resistant mutations. DA - 2024-01 DB - ResearchSpace DP - CSIR J1 - Proceedings of SPIE Conference 12840 - Optical Interactions with Tissue and Cells XXXV, San Francisco, California, United States, 27-28 January 2024 KW - Multidrug-resistance KW - Tuberculosis KW - TB KW - Sensor chip KW - Probes KW - Hybridization KW - Surface Plasmon resonance LK - https://researchspace.csir.co.za PY - 2024 SM - 9781510669390 SM - 1605-7422 T1 - Biosensing multidrug-resistant TB genes using SPR TI - Biosensing multidrug-resistant TB genes using SPR UR - http://hdl.handle.net/10204/13675 ER - en_ZA
dc.identifier.worklist 27818 en_US


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