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Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system

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dc.contributor.author Thobakgale, Setumo L
dc.contributor.author Ombinda-Lemboumba, Saturnin
dc.contributor.author Mthunzi-Kufa, Patience
dc.date.accessioned 2020-03-19T08:00:59Z
dc.date.available 2020-03-19T08:00:59Z
dc.date.issued 2019-02
dc.identifier.citation Thobakgale, S.L., Ombinda-Lemboumba, S. & Mthunzi-Kufa, P. 2019. Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system. In: Proceedings of SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 1088416, San Francisco, California, United States, 22 February 2019 en_US
dc.identifier.uri https://doi.org/10.1117/12.2509839
dc.identifier.uri https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10885/108850N/Investigation-and-calibration-of-non-essential-amino-acids-using-a/10.1117/12.2509839.full?sessionGUID=02bc9993-ced2-68be-ad49-a28531ab0d3a&webSyncID=6ec7a49d-ab6a-0cba-d059-329ad883c9d9&sessionGUID=02bc9993-ced2-68be-ad49-a28531ab0d3a&SSO=1
dc.identifier.uri https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10885/2509839/Investigation-and-calibration-of-non-essential-amino-acids-using-a/10.1117/12.2509839.full
dc.identifier.uri http://hdl.handle.net/10204/11348
dc.description Presented in: Proceedings of SPIE 10885, Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics, 108850N, San Francisco, California, United States, 20 February 2019. Due to copyright restrictions, the attached PDF file contains the abstract of the full-text item. For access to the full-text item, please consult the publisher's website. en_US
dc.description.abstract Nonessential amino acids (NEAAs) are the building blocks for producing proteins of various functionalities within living systems. For example, from NEAAs, humans produce proteins that are important for cell repair, cell division and synthesis of hormones paramount to maintaining a healthy body. Understanding the chemical properties of NEAAs can therefore allow a thorough investigation of biological processes in the cases of ailing individuals. Raman spectroscopy has become a powerful technique for characterizing various organic compounds such as NEAAs, relying on their unique light scattering properties. In this study we present a custom built Raman Spectroscopy system which was used to investigate and calibrate a group of NEAAs through a comparison of peak area versus concentration. The results show that various regions of interest within the sample mixtures exhibit an increase in signal intensity when the concentration is increased. This is in agreement with current literature on the parameters that effect Raman systems such as concentration. Secondly, it was observed that 0.1 mM of NEAAs was the current detection limit of the system in terms of number of significant peaks produced. As a result, it is important that future work includes incorporating nanomaterials as sample scaffolds for signal enhancement to improve sensitivity. Much of this work is intended towards producing a point-of-care diagnostic tool for analysis of cancer agents. en_US
dc.language.iso en en_US
dc.publisher SPIE en_US
dc.relation.ispartofseries Workflow;22754
dc.subject Nonessential amino acids en_US
dc.subject NEEAs en_US
dc.subject Raman spectroscopy systems en_US
dc.subject Point-of-care diagnostics en_US
dc.title Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system en_US
dc.type Conference Presentation en_US


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