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An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles

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dc.contributor.author Stander, BA
dc.contributor.author van Vollenstee, FA
dc.contributor.author Kallmeyer, K
dc.contributor.author Potgieter, M
dc.contributor.author Joubert, A
dc.contributor.author Swanepoel, Andri
dc.contributor.author Kotzé-Jacobs, Lara
dc.contributor.author Moolman, S
dc.contributor.author Pepper, MS
dc.date.accessioned 2018-08-15T07:20:42Z
dc.date.available 2018-08-15T07:20:42Z
dc.date.issued 2018-07
dc.identifier.citation Stander, B.A. et al. 2018. An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles. PLoS ONE, vol. 13(7): e0198248 en_US
dc.identifier.issn 1932-6203
dc.identifier.uri https://doi.org/10.1371/journal.pone.0198248
dc.identifier.uri http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198248
dc.identifier.uri http://hdl.handle.net/10204/10362
dc.description Open access article published in PLoS ONE, vol. 13(7): e0198248 en_US
dc.description.abstract The field of dermal fillers is evolving rapidly and numerous products are currently on the market. Biodegradable polymers such as polycaprolactone (PCL) have been found to be compatible with several body tissues, and this makes them an ideal material for dermal filling purposes. Hollow PCL spheres were developed by the Council for Scientific and Industrial Research (CSIR) to serve both as an anchor point and a "tissue harbour" for cells. Particles were tested for cytotoxicity and cell adherence using mouse embryo fibroblasts (MEF). MEFs adhered to the particles and no significant toxic effects were observed based on morphology, cell growth, cell viability and cell cycle analysis, suggesting that the particles are suitable candidates for cell delivery systems in an in vivo setting. The objective of providing a "tissue harbour" was however not realized, as cells did not preferentially migrate into the ported particles. In vivo studies were conducted in BALB/c mice into whom particles were introduced at the level of the hypodermis. Mice injected with PCL particles (ported and nonported; with or without MEFs) showed evidence of local inflammation and increased adipogenesis at the site of injection, as well as a systemic inflammatory response. These effects were also observed in mice that received apparently inert (polystyrene) particles. Ported PCL particles can therefore act as a cell delivery system and through their ability to induce adipogenesis, may also serve as a dermal bulking agent. en_US
dc.language.iso en en_US
dc.publisher PLOS en_US
dc.relation.ispartofseries Worklist;21189
dc.subject polycaprolactone cell-delivery particles en_US
dc.subject dermal fillers en_US
dc.subject biodegradable polymers en_US
dc.subject MEFs en_US
dc.subject mouse embryo fibroblasts en_US
dc.subject dermal bulking agent en_US
dc.title An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles en_US
dc.type Article en_US


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