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Browsing by Author "Thobakgale, Setumo L"

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Chemical sensor nanotechnology in pharmaceutical drug research

Author: Thobakgale, Setumo L ; Ombinda-Lemboumba, Saturnin ; Mthunzi-Kufa, Patience Date: Aug 2022 The increase in demand for pharmaceutical treatments due to pandemic-related illnesses has created a need for improved quality control in drug manufacturing. Understanding the physical, biological, and chemical properties of APIs is an important ... Read more
Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study

Author: Thobakgale, Setumo L ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Maaza, M ; Mthunzi-Kufa, Patience Date: Jan 2017 Embryonic stem cells have great promise in regenerative medicine because of their ability to self-renew and differentiate into various cell types. Delivery of therapeutic genes into cells has already been achieved using of chemical agents and ... Read more
Exploring optical spectroscopic techniques and nanomaterials for virus detection

Author: Manoto, Sello L ; El-Hussein, A ; Malabi, Rudzani ; Thobakgale, Setumo L ; Ombinda-Lemboumba, Saturnin ; Attia, YA ; Kasem, MA ; Mthunzi-Kufa, Patience Date: Aug 2020 Viral infections pose significant health challenges globally by affecting millions of people worldwide and consequently resulting in a negative impact on both socioeconomic development and health. Corona virus disease 2019 (COVID-19) is a ... Read more
Femtosecond laser assisted photo-transfection and differentiation of mouse embryonic stem cells

Author: Thobakgale, Setumo L ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Maaza, M ; Mthunzi-Kufa, Patience Date: Jan 2018 In tissue engineering research, stem cells have been used as starting material in the synthesis of mammalian cells for the treatment of various cell based diseases. This is done by manipulating the DNA content of the cells to induce a specific ... Read more
Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy

Author: Thobakgale, Setumo L ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Mthunzi-Kufa, Patience Date: Feb 2020 Polypeptide gelatine has been used extensively in microbiology to enhance cellular adhesion and growth. Likewise, fabrication of biochemical sensors using a variety of organic material and nanomaterials is a growing research area particularly ... Read more
Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system

Author: Thobakgale, Setumo L ; Ombinda-Lemboumba, Saturnin ; Mthunzi-Kufa, Patience Date: Feb 2019 Nonessential amino acids (NEAAs) are the building blocks for producing proteins of various functionalities within living systems. For example, from NEAAs, humans produce proteins that are important for cell repair, cell division and synthesis ... Read more
Investigation of HIV-1 infected and uninfected cells using the optical trapping technique

Author: Ombinda-Lemboumba, Saturnin ; Malabi, Rudzani ; Lugongolo, Masixole Y ; Thobakgale, Setumo L ; Manoto, Sello L ; Mthunzi-Kufa, Patience Date: Feb 2017 Optical trapping has emerged as an essential tool for manipulating single biological material and performing sophisticated spectroscopy analysis on individual cell. The optical trapping technique has been used to grab and immobilize cells ... Read more
Investigation of HIV-1 infected and uninfected cells using the optical trapping technique

Author: Ombinda-Lemboumba, Saturnin ; Malabi, Rudzani ; Lugongolo, Masixole Y ; Thobakgale, Setumo L ; Manoto, Sello L ; Mthunzi-Kufa, Patience Date: Feb 2017 Optical trapping has emerged as an essential tool for manipulating single biological material and performing sophisticated spectroscopy analysis on individual cell. The optical trapping technique has been used to grab and immobilize cells ... Read more
Label-free detection of mutations in the HIV genome using a surface plasmon resonance biosensor

Author: Lugongolo, Masixole Y ; Manoto, Sello L ; Maphanga, Charles P ; Ombinda-Lemboumba, Saturnin ; Thobakgale, Setumo L ; Mthunzi-Kufa, Patience Date: Mar 2021 Surface plasmon resonance (SPR) biosensors are optical materials that measure changes in the refractive index as they monitor non-covalent molecular interactions in real time. These utilise a label free analytical approach, which does not ... Read more
Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement

Author: Malabi, Rudzani ; Lugongolo, Masixole Y ; Thobakgale, Setumo L ; Manoto, Sello L ; Mthunzi-Kufa, Patience ; Ombinda-Lemboumba, Saturnin Date: Feb 2019 Transmission measurement has been perceived as a potential candidate for label-free investigation of biological material. It is a real-time, label-free and non-invasive optical detection technique that has found wide applications in pharmaceutical ... Read more
A molecular study of aspirin and tenofovir using gold/dextran nanocomposites and surface-enhanced raman spectroscopy

Author: Thobakgale, Setumo L ; Ombinda-Lemboumba, Saturnin ; Mthunzi-Kufa, Patience Date: Apr 2022 In this study, we show how surface enhanced Raman spectroscopy (SERS) can be used to monitor the molecular behaviour of aspirin and tenofovir as a means of screening medication for quality control purposes. Gold-coated slides combined with ... Read more
Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres

Author: Thobakgale, Setumo L ; Ombinda-Lemboumba, Saturnin ; Mthunzi-Kufa, Patience ; Malabi, Rudzani Date: Feb 2019 In the recent decade, Raman spectroscopy has played a key role in photonics as a powerful method suited for detection, diagnosis and screening applications across various industrial fields. In this work, we propose a home built Raman Spectroscopy ... Read more
Photo-transfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses

Author: Thobakgale, Setumo L Date: Jan 2017 This presentation is about the photo-transfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses. It outlines the background on embryonic stem cells (ES) and phototransfection. Read more
Photo-transfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses

Author: Thobakgale, Setumo L Date: Jan 2017 This presentation is about the photo-transfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses. It outlines the background on embryonic stem cells (ES) and phototransfection. Read more
Phototransfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses

Author: Thobakgale, Setumo L ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Maaza, M ; Mthunzi-Kufa, Patience Date: Feb 2017 Cellular manipulation by delivery of molecules into cells has been applied extensively in tissue engineering research for medical applications. The different molecular delivery techniques used range from viral and chemical agents to physical ... Read more
Raman spectroscopy and gold thin film for biosensing and detection

Author: Ombinda-Lemboumba, Saturnin ; Manoto, Sello L ; Maphanga, Charles P ; Malabi, Rudzani ; Thobakgale, Setumo L ; Lugongolo, Masixole Y ; Mthunzi-Kufa, Patience Date: Feb 2020 Raman spectroscopy is commonly used for sample characterization in biology because vibrational information is very specific to the chemical bonds in molecules. This makes it an attractive approach for identification of biological materials ... Read more
Surface enhanced raman spectroscopy on polymer-graphene oxide scaffolds for drug screening applications

Author: Thobakgale, Setumo L ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Mthunzi-Kufa, Patience Date: May 2020 Surface enhanced Raman spectroscopy (SERS) has evolved to be a powerful analytical tool for investigating molecular properties of various types of samples. Literature has shown SERS capabilities in both qualitative and quantitative analysis ... Read more
Therapeutic strategies to fight HIV-1 latency: progress and challenges

Author: Manoto, Sello L ; Thobakgale, Setumo L ; Malabi, Khorommbi P ; Maphanga, Charles P ; Ombinda-Lemboumba, Saturnin ; Mthunzi-Kufa, Patience Date: Oct 2017 The life-long persistence of human immunodeficiency virus type-1 (HIV-1) in latent reservoirs is a major hurdle in the eradication of HIV-1, even though highly active antiretroviral therapy (HAART) can be effective in reducing the plasma HIV-1 ... Read more

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