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Browsing by Author "Stoychev, Stoyan H"

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Biochemical pulldown of mRNAs and long noncoding RNAs from cellular lysates coupled with mass spectrometry to identify protein binding partners

Author: Savulescu, AF ; Stoychev, Stoyan H ; Mamputha, Sipho ; Mhlanga, MM Date: Jun 2020 RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition ... Read more
Boar seminal plasma proteomic profiling for biomarker discovery

Author: Mokwena, Pateswana W ; Stoychev, Stoyan H ; Buthelezi, Sindisiwe G ; Naicker, Previn ; Ramukhithi, FV ; Lehloenya, KC Date: Dec 2022 As an important technology in the swine industry, artificial insemination is of great significance in the preservation of elite breeds and the improvement of the utilisation rate of elite breeds, which makes the quality of preserved pig semen ... Read more
Challenges and opportunities for biological mass spectrometry core facilities in the developing world

Author: Bell, L ; Calder, B ; Hiller, R ; Klein, A ; Soares, NC ; Stoychev, Stoyan H ; Vorster, BC ; Tabb, DL Date: Apr 2018 The developing world is seeing rapid growth in the availability of biological mass spectrometry (MS), particularly through core facilities. As proteomics and metabolomics becomes locally feasible for investigators in these nations, application ... Read more
Characterisation of African elephant beta casein and its relevance to the chemistry of caseins and casein micelles

Author: Madende, M ; Kemp, G ; Stoychev, Stoyan H ; Osthoff, G Date: Oct 2018 Copyright: 2018 Elsevier. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. Read more
Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197

Author: Roth, Robyn L ; Van Zyl, Petrus J ; Tsekoa, Tsepo L ; Stoychev, Stoyan H ; Mamputha, Sipho ; Buthelezi, Sindisiwe G ; Crampton, Michael C Date: Apr 2017 The aim of this article is to investigate the production of soluble cross-reacting material 197 (CRM(sub197)) in Escherichia coli, a safe and effective T-cell-dependent protein carrier for polysaccharides used in the manufacture and application ... Read more
Covalent binding of human two-domain CD4 to an HIV-1 subtype C SOSIP.664 trimer modulates its structural dynamics

Author: Tumba, NL ; Naicker, Previn ; Stoychev, Stoyan H ; Killick, MA ; Owen, GR ; Papathanasopoulos, MA Date: Jul 2022 The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) mediates host cell infection by binding to the cellular receptor CD4. Recombinant Env bound to CD4 has been explored for its potential as an HIV vaccine immunogen as ... Read more
Differential inhibition of adenylylated and deadenylylated forms of M. tuberculosis glutamine synthetase as a drug discovery platform

Author: Theron, Anjo ; Roth, Robyn L ; Hoppe, H ; Parkinson, C ; Van der Westhuyzen, Christiaan W ; Stoychev, Stoyan H ; Wiid, I ; Pietersen, RD ; Baker, B ; Kenyon, CP Date: Oct 2017 Glutamine synthetase is a ubiquitous central enzyme in nitrogen metabolism that is controlled by up to four regulatory mechanisms, including adenylylation of some or all of the twelve subunits by adenylyl transferase. It is considered a ... Read more
Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous

Author: Chhiba, Varsha P ; Mathiba, Kgama ; Van der Westhuyzen, Christiaan W ; Steenkamp, Paul A ; Rashamuse, JK ; Stoychev, Stoyan H ; Bode, ML ; Brady, D Date: Dec 2018 Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase ... Read more
The effects of mutating Tyr9 and Arg15 on the structure, stability, conformational dynamics and mechanism of GSTA3-3

Author: Robertson, GJ ; Stoychev, Stoyan H ; Sayed, Y ; Achilonu, I ; Dirr, HW Date: Mar 2017 Glutathione S-transferase A3-3 is the most catalytically efficient steroid isomerase enzyme known in humans, transforming Δ5-androstene-3-17-dione into Δ4-androstene-3-17-dione. GSTA3-3 catalyzes this reaction with ten-fold greater efficiency ... Read more
Efficient in vitro and in vivo activity of glyco-engineered plant-produced rabies monoclonal antibodies E559 and 62-71-3

Author: Tsekoa, Tsepo L ; Lotter-Stark, Therese ; Buthelezi, Sindisiwe G ; Chakauya, Ereck ; Stoychev, Stoyan H ; Chikwamba, Rachel K Date: Jul 2016 Rabies is a neglected zoonotic disease that has no effective treatment after onset of illness. However the disease can be prevented effectively by prompt administration of post exposure prophylaxis which includes administration of passive ... Read more c b
The forkhead domain hinge-loop plays a pivotal role in DNA binding and transcriptional activity of FOXP2

Author: Morris, G ; Stoychev, Stoyan H ; Naicker, Previn ; Dirr, HW ; Fanucchi, Stephanie Date: Jul 2018 Forkhead box (FOX) proteins are a ubiquitously expressed family of transcription factors that regulate the development and differentiation of a wide range of tissues in animals. The FOXP subfamily members are the only known FOX proteins capable ... Read more
Gel and gel-free approaches for the quantitative characterisation of complex protein mixtures

Author: Buthelezi, Sindisiwe G ; Tsekoa, Tsepo L ; Stoychev, Stoyan H ; Mancama, Dalubuhle T ; Blackburn, J Date: Oct 2012 The research aims to establish a reliable set of methods for profiling proteins in a complex mixture in order to allow for the mining of low abundant species. Read more
Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica

Author: Kahari, D ; Koorsen, G ; Stoychev, Stoyan H ; Chipeta, Z ; Labuschagne, M ; Van Zyl, P ; Nthangeni, B Date: Nov 2008 The CSIR Biosciences researchers are developing a platform for robust techniques for glyco-profiling of therapeutic glycoproteins. The extracellular lipase (Lip2) (Aloulou, A et al;2007) was used as a model in establishing and evaluating ... Read more
Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum

Author: Mehlo, L ; Mbambo, Z ; Bado, S ; Lin, J ; Moagi, SM ; Buthelezi, Sindisiwe G ; Stoychev, Stoyan H ; Chikwamba, Rachel K Date: Sep 2013 Physical and biochemical analysis of protein polymorphisms in seed storage proteins of a mutant population of sorghum revealed a mutant with redirected accumulation of kafirin proteins in the germ. The change in storage proteins was accompanied ... Read more
Inhibition of Plasmodium falciparum spermidine synthase indicates unique perturbation-specific effects in the transcriptome and proteome

Author: Becker, J ; Mtwisha, L ; Crampton, BG ; Stoychev, Stoyan H ; Van Brummelen, A ; Louw, A ; Birkholtz, L ; Mancama, Dalubuhle T Date: Mar 2009 Antiplasmodial drug discovery efforts are hampered by the lack of an extensive set of novel, validated drug targets. A requirement of these targets (or the pathways in which they function) is that they prove essential for parasite survival. ... Read more
JNK1ß1 is phosphorylated during expression in E. coli and in vitro by MKK4 at three identical novel sites

Author: Owen, GR ; Stoychev, Stoyan H ; Achilonu, I ; Dirr, HW Date: Mar 2013 JNK1 is activated by phosphorylation of the canonical T183 and Y185 residues, modifications that are catalysed typically by the upstream eukaryotic kinases MKK4 and MKK7. Nonetheless, the exact sites at which the most abundant JNK variant, ... Read more
Open search unveils modification patterns in formalin-fixed, paraffin-embedded thermo HCD and SCIEX TripleTOF shotgun proteomes

Author: Tabb, DL ; Murugan, BD ; Okendo, J ; Nair, O ; Blackburn, JM ; Buthelezi, Sindisiwe G ; Stoychev, Stoyan H Date: Feb 2020 The application of database search algorithms with very wide precursor mass tolerances for the “Open Search” paradigm has brought new efforts at post-translational modification discovery in shotgun proteomes. This approach has motivated the ... Read more
Phosphorylation- and nucleotide-binding-induced changes to the stability and hydrogen exchange patterns of JNK1ß1 provide insight into its mechanisms of activation

Author: Owen, GR ; Stoychev, Stoyan H ; Achilonu, I ; Dirr, HW Date: Oct 2014 Many studies have characterized how changes to the stability and internal motions of a protein during activation can contribute to their catalytic function, even when structural changes cannot be observed. Here, unfolding studies and ... Read more
Plant-based production of highly potent anti-HIV antibodies with engineered posttranslational modifications

Author: Singh, Advaita Acarya ; Pooe, O ; Kwezi, Lusisizwe ; Lotter-Stark, T ; Stoychev, Stoyan H ; Alexandra, Kabamba B ; Gerber, Isak B ; Bhiman, JN ; Vorster, J ; Pauly, M ; Zeitlin, L ; Whaley, K ; Mach, L ; Steinkellner, H ; Morris, L ; Tsekoa, Tsepo L ; Chikwamba, Rachel K Date: Apr 2020 Broadly neutralising antibodies (bNAbs) against human immunodeficiency virus type 1 (HIV-1), such as CAP256-VRC26 are being developed for HIV prevention and treatment. These Abs carry a unique but crucial post-translational modification (PTM), ... Read more
Plasmodium falciparum spermidine synthase inhibition results in unique perturbation-specific effects observed on transcript, protein and metabolite levels

Author: Becker, JVW ; Mtwisha, L ; Crampton, BG ; Stoychev, Stoyan H ; Van Brummelen, AC ; Reeksting, S ; Louw, AI ; Birkholtz, LM ; Mancama, Dalubuhle T Date: Jan 2010 This study details the malaria parasite's response to PfSpdSyn inhibition on the transcriptomic, proteomic and metabolic levels. The results corroborate and significantly expand previous functional genomics studies relating to polyamine ... Read more

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