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Browsing by Author "Steenkamp, Paul A"

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Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation

Author: Ncube, EN ; Steenkamp, Paul A ; Madala, NE ; Dubery, IA Date: Jul 2016 Exogenous application of synthetic and natural elicitors of plant defence has been shown to result in mass production of secondary metabolites with nutraceuticals properties in cultured cells. In particular, salicylic acid (SA) treatment has ... Read more
Conversation on data mining strategies in LC-MS untargeted metabolomics: pre-processing and pre-treatment steps

Author: Tugizimana, F ; Steenkamp, Paul A ; Piater, LA ; Dubery, IA Date: Nov 2016 Untargeted metabolomic studies generate information-rich, high-dimensional, and complex datasets that remain challenging to handle and fully exploit. Despite the remarkable progress in the development of tools and algorithms, the “exhaustive” ... Read more c b
Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous

Author: Chhiba, Varsha P ; Mathiba, Kgama ; Van der Westhuyzen, Christiaan W ; Steenkamp, Paul A ; Rashamuse, JK ; Stoychev, Stoyan H ; Bode, ML ; Brady, D Date: Dec 2018 Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase ... Read more
Distribution patterns of flavonoids from three Momordica species by ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry: a metabolomic profiling approach

Author: Madala, NE ; Piater, L ; Dubery, I ; Steenkamp, Paul A Date: Aug 2016 Plants from the Momordica genus, Curcubitaceae, are used for several purposes, especially for their nutritional and medicinal properties. Commonly known as bitter gourds, melon and cucumber, these plants are characterized by a bitter taste ... Read more
Effect of geometrical isomerism of 3,5-dicaffeoylquinic acid on its binding affinity to HIV-integrase enzyme: a molecular docking study

Author: Makola, MM ; Dubery, IA ; Koorsen, G ; Steenkamp, Paul A ; Kabanda, MM ; Du Preez, LL ; Madala, NE Date: 2016 A potent plant-derived HIV-1 inhibitor, 3,5-dicaffeoylquinic acid (diCQA), has been shown to undergo isomerisation upon UV exposure where the naturally occurring, 3(suptrans), 5(suptrans)-diCQA isomer gives rise to the 3(supcis), 5(suptrans)-diCQA, ... Read more c b
Identification of hydroxylcinnamoyl tartaric acid esters in Bidens pilosa by UPLC-tandem mass spectrometry

Author: Khoza, BS ; Gbashi, S ; Steenkamp, Paul A ; Njobe, PB ; Madala, NE Date: Mar 2016 Bidens pilosa is a medicinal plant used for the treatment of several physiological illnesses. In South Africa, as the case may be for other African countries, this plant is equally consumed as a vegetable. In the current study, pressurized ... Read more
Influence of the geometric isomers on the radical scavenging properties of 3,5-dica®eoylquinic acid: A DFT study in vacuo and in solution

Author: Makola, MM ; Madala, NE ; Dubery, IA ; Steenkamp, Paul A ; Kabanda, MM Date: Sep 2016 3,5-Dica®eoylquinic acid (diCQA) is a part of the chlorogenic acid group of compounds, largely isolated from food sources and possessing potent antioxidant activity. Only the trans–trans isomer exists in nature, however, abiotic stresses, ... Read more
Lipopolysaccharide-induced metabolome signature in Arabidopsis thaliana reveals dynamic reprogramming of Phytoalexin and Phytoanticipin pathways

Author: Finnegan, T ; Steenkamp, Paul A ; Piater, LA ; Dubery, IA Date: Sep 2016 Lipopolysaccharides (LPSs), as MAMP molecules, trigger the activation of signal transduction pathways involved in defence. Currently, plant metabolomics is providing new dimensions into understanding the intracellular adaptive responses to ... Read more c b
Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid

Author: Mhlongo, MI ; Piater, LA ; Steenkamp, Paul A ; Madala, NE ; Dubery, IA Date: Jan 2015 Previous studies suggest that only transisomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated tobacco leaves exposed to ... Read more
A one-pot laccase-catalysed synthesis of coumestan derivatives and their anticancer activity

Author: Qwebani-Ogunleye, Tozama ; Kolesnikova, Natalia I ; Steenkamp, Paul A ; De Koning, CB ; Brady, D ; Wellington, Kevin W Date: Dec 2016 Suberase®, a commercial laccase from Novozymes, was used to catalyse the synthesis of coumestans. The yields, in some cases, were similar to or better than that obtained by other enzymatic, chemical or electrochemical syntheses. The compounds ... Read more
Perturbation of pharmacologically relevant polyphenolic compounds in Moringa oleifera against photo-oxidative damages imposed by gamma radiation

Author: Ramabulana, T ; Mavunda, RD ; Steenkamp, Paul A ; Piater, LA ; Dubery, IA ; Madala, NE Date: Mar 2016 Oxidative stress is a physiological state associated with almost all biotic and abiotic stresses in plants. This phenomenon occurs due to imbalances which result from the overproduction of reactive oxygen species (ROS). Plants, however, have ... Read more
Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Author: Makola, MM ; Steenkamp, Paul A ; Dubery, IA ; Kabanda, MM ; Madala, NE Date: Mar 2016 Caffeoylquinic acid (CQA) derivatives are a group of structurally diverse phytochemicals that have attracted attention due to their many health benefits. The structural diversity of these molecules is due in part to the presence of regio- and ... Read more
Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry

Author: Gbashi, S ; Njobeh, P ; Steenkamp, Paul A ; Madala, N Date: 2017 The need for greener extraction procedures that are quick and efficient has prompted the evolution of pressurized hot water extraction (PHWE). Here, the extraction of flavonoids from Bidens pilosa was demonstrated using PHWE at 50°C, 100°C ... Read more
Profiling of altered metabolomic states in Nicotiana tabacum cells induced by priming agents

Author: Mhlongo, MI ; Steenkamp, Paul A ; Piater, LA ; Madala, NE ; Dubery, IA Date: Oct 2016 Metabolomics has developed into a valuable tool for advancing our understanding of plant metabolism. Plant innate immune defenses can be activated and enhanced so that, subsequent to being pre-sensitized, plants are able to launch a stronger ... Read more
Profiling of botanical extracts for authentication, detection of adulteration and quality control using UPLC-QTOF-MS

Author: Steenkamp, Paul A ; Steenkamp, Lucia H ; Mancama, Dalubuhle T Date: Jan 2018 Herbs and plant extracts are very popular and are being used by all cultures for healthcare and as food supplements. The correct identification of the required plant material is vital for formulation of final formulas for use by mankind. The ... Read more
Stimulatory effects of acibenzolar-s-methyl on chlorogenic acids biosynthesis in Centella asiatica cells

Author: Ncube, EN ; Steenkamp, Paul A ; Madala, NE ; Dubery, IA Date: Sep 2016 Centella asiatica is a perrenial herb that grows in tropical regions with numerous medicinal properties mostly attributed to the presence of pentacyclic triterpenoids. Interestingly, this plant also possess a significant amount of ... Read more
Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells

Author: Mhlongo, MI ; Tugizimana, F ; Piater, LA ; Steenkamp, Paul A ; Madala, NE ; Dubery, IA Date: Jan 2017 To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a ... Read more
UPLC-MS profiling, identification of major peaks and comparison of Harpagophytum procumbens extracts from different locations

Author: Steenkamp, Paul A ; Steenkamp, Lucia H Date: Aug 2019 Organic extracts (methanol:acetonitrile (1:1 v/v)) of the dried and grounded secondary tubers of Devil's Claw, Harpagophytum procumbens (Burch.) DC. ex Meisn., produced extracts rich in iridoid glucosides. An UPLC-MS profiling method was ... Read more
Variation in pharmacologically potent rutinoside-bearing flavonoids amongst twelve Moringa oleifera Lam. cultivars

Author: Makita, C ; Madala, NE ; Cukrowska, A ; Abdelgadir, H ; Chimuka, L ; Steenkamp, Paul A ; Ndhlala, AR Date: Sep 2017 Recent evidence has shown that consumption of plants rich in phenolic compounds has health-promoting benefits. Amongst these plants, include Moringa oleifera Lam. (Moringaceae) which has gained significant attention due to its high contingency ... Read more

Now showing items 1-19 of 19

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