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Browsing by Author "Rashamuse, KJ"

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Characterisation of two bifunctional cellulase-xylanase enzymes isolated from a bovine rumen metagenome library

Author: Rashamuse, KJ ; Visser, Daniel F ; Hennessy, F ; Kemp, J ; Van der Merwe, MP ; Badenhorst, J ; Ronneburg, T ; Francis-Pope, R ; Brady, D Date: Feb 2013 Ruminant digestive tract microbes hydrolyse plant biomass, and the application of metagenomic techniques can provide good coverage of their glycosyl hydrolase enzymes. A metagenomic library of circa 70,000 fosmids was constructed from bacterial ... Read more
Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
Enzymatic recovery of platinum (IV) from industrial wastewater using a biosulphidogenic hydrogenase

Author: Rashamuse, KJ ; Mutambanengwe, CCZ ; Whiteley, CG Date: Apr 2008 It has been established that dissolved heavy metals escaping into the environment pose a serious health hazard. As a result, there is an urgent need for controlling metal emissions into the environment. The aims of this study were to purify ... Read more
Molecular characterization of a novel family VIII esterase from burkholderia multivorans UWC10

Author: Rashamuse, KJ ; Burton, SG ; Stanfford, WHL ; Cowan, DA Date: Feb 2007 An esterase producing Burkholderia multivorans UWC10 strain was isolated by culture enrichment. A shotgun library of B. multivorans UWC10 genomic DNA was screened for esterase activity and a recombinant clone conferring an esterolytic phenotype ... Read more
Novel recombinant ethyl ferulate esterase from Burkholderia multivorans

Author: Rashamuse, KJ ; Burton, SG ; Cowan, DA Date: Nov 2007 Isolation and identification of bacterial isolates with specific ferulic acid (FA) esterase activity and cloning of a gene encoding activity. A micro-organism with ethyl ferulate hydrolysing (EFH) activity was isolated by culture enrichment ... Read more

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Browsing by Author "Rashamuse, KJ"

Browsing by Author "Rashamuse, KJ"

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